The invention discloses a damping tensioning structure for a glass lifter stay wire. The damping tensioning structure comprises a first support body, a second support body and a damping spring. An opening containing groove is formed in the first support body, and a through assembling channel is formed in the bottom of the containing groove. The second support body is assembled in the containing groove, the first end of the second support body penetrates the assembling channel and extends outwards, and the outer contour face of the second end of the second support body is matched with the inner contour face of the containing groove. An air cavity is formed between the end face of the second end of the second support body and the inner wall of the containing groove, a through air via hole is formed in the second end of the second support body, and the air via hole communicates with the air cavity. The damping spring is assembled in the air cavity. By means of the damping tensioning structure for the glass lifter stay wire, extension of the stay wire is balanced, and jittering or vibrating in an existing scheme is eliminated through resistance generated by air compressing and blowing-down.