The invention discloses a keratinase mutant with increased thermal stability and application thereof, which belong to the technical field of industrial biotechnology. Asparagine at the 181st site, tyrosine at the 218th site and serine at the 236th site in keratinase derived from Brevibacillus parabrevis CGMCC No.10798 undergo site-directed mutation modification, moreover, the sites are randomly combined, and thereby the combined mutant is prepared. The thermal stability of keratinase is remarkably increased by 1 to 4.09 times, and good theoretical value and application prospect are achieved.