The invention discloses a modeling method of a non-rodent animal model for studying intestinal flora. A male rhesus monkey of 12-months old is taken as a laboratory animal and fed with mixed antibiotic for 21 days to clear symbiotic intestinal flora in the body. The antibiotic includes neomycin, streptomycin, ampicillin sodium, kanamycin, metronidazole and vancomycin; different antibiotic formulasare adopted for the first 10 days and later 11 days of feeding. The modeling result is verified by combining a Realtime-PCR method and an amplicon sequencing method. The method disclosed by the invention has the advantages of simplicity and easiness in implementation, short time, high repeatability, low death rate and stable model. The method has an important application value in the treatment offlora-related metabolic diseases, inflammatory bowel diseases and infectious diseases, evaluation of intestinal flora in the immune system, development of new medicines and the like.