The invention discloses a tea tree LAI and nitrogen content estimation method based on hyperspectral images. The method comprises acquiring multiple tea plants as test samples, carrying out correlation and regression analysis on LAI, nitrogen content, hyperspectral position variable, hyperspectral area variable and vegetation index variable of a tea tree as a test sample, building a final tea treeLAI estimation model and a final tea tree nitrogen content estimation model, acquiring hyperspectral position variable, hyperspectral area variable and vegetation index variable of the tea tree needing the LAI and nitrogen content test, respectively inputting the above variables of the tea tree into the final tea tree LAI estimation model and the final tea tree nitrogen content estimation model and calculating the LAI and nitrogen content of the tea tree. The method has the advantages of real-time estimation of LAI and nitrogen content of a tea tree, fastness and no damage.