The invention provides a preparing method of a serial olefinic-terpene-type compound, and belongs to the technical field of compound preparation through co-culture of edible and medicinal fungi. Trametes robimiophila Murr. and pleurotus ostreatus are co-cultured to obtain the serial olefinic-terpene-type compound which has a novel structural feature, and candida albicans, cryptococcus neoformans,staphylococcus aureus, valsa ceratosperma and a human lung cancer cell line A549 can be effectively inhibited. As is shown by data of the embodiment, the serial olefinic-terpene-type compound has a certain capability of inhibiting candida albicans, cryptococcus neoformans, valsa ceratosperma, and staphylococcus aureus and has a good effect of inhibiting the human lung cancer cell line A549.