The invention discloses an acylthiourea compound, a preparation method thereof and application of the acylthiourea compound in resisting influenza A viruses. Substituted aromatic formyl chloride compounds, amine thiocyanate and substituted aromatic heterocyclic amine compounds are used as raw materials, and series I acyl thiourea compounds are prepared through a one-step reaction. In addition, V-17 in the series I acyl thiourea compounds is used as a raw material, and is subjected to an amide reaction with different acyl chloride compounds to obtain the series II acyl thiourea compounds. The acylthiourea compound or the pharmacologically or physiologically acceptable salt thereof has anti-influenza A virus activity and low toxicity to cells, can be developed as a new anti-influenza virus drug, and has a wide application prospect.