The invention discloses a prefabricated box culvert connection structure resistant to longitudinal settlement. The connection structure includes a reinforced concrete structure for connecting each section of prefabricated box culverts to form an integral whole, holes for concrete to be poured are respectively formed in a bottom plate of each prefabricated box culvert, the reinforced concrete structure includes a reinforced framework and concrete, the reinforced framework includes longitudinal tensile reinforcement and each group of vertical reinforcement correspondingly located in the holes ofthe bottom plate of each prefabricated box culvert, and each group of the vertical reinforcement is respectively connected by the longitudinal tensile reinforcement. According to the prefabricated box culvert connection structure, connection is added at the bottom of each ring box culvert structure, integrity of the prefabricated box culvert structure is enhanced, and thus requirements of longitudinal settlement resistance are met.