The invention discloses a calibration method of a binocular vision measurement technology, and the method comprises the following steps of 1, separately fixing two sets of camera assemblies on a crossbeam, setting a target, adjusting the angles between the camera assemblies and the target, and focusing; 2, inquiring and reading the working environment parameters of the camera assemblies; 3, adjusting the illuminance of the image acquisition areas, corresponding to the camera assemblies, on the target to enable the illuminance of the corresponding image acquisition areas on the target to reacha set value; 4, controlling the camera assemblies to acquire a target image, observing whether the image is clear and complete, and if the image is not clear and complete, adjusting until the clear and complete image is obtained; and 5, adjusting the position and the angle of the target, and carrying out image acquisition respectively until the clear and complete images are obtained at differentpositions and different angles, thereby completing calibration. Through calibration, the imaging definition of a to-be-measured moving object can be improved, and the measurement accuracy and precision are improved.