A clamping positioning device for sleepers comprises: a main tube and multiple clamping units which are distributed on the main tube; the multiple clamping units include holders and ribs which are fixed on the main tube; a sliding slide set outside of the main tube is arranged between the holders and the ribs. The opposite sides of the holders and the slide match with the shape of both sides of the sleeper. The sleeper and the ribs which are clamping on both sides can be arranged with locking mechanism which can fix the position of the slide. A downward support plate is arranged on the part of the main tube which is between the slide and the holder. The main tube of the positioning clamping device supports each sleeper through each support plate. The holder and the slide of each clamping unit are located on both sides of the sleeper in order to promote the slide along the main tube. The device also makes use of the locking mechanism to fix the slide and the main tube. The holder and the slide can clamp the sleeper from both sides. A number of clamping units on the main tube can clamp and locate a number of sleepers. A number of sleepers are clamped and fixed as a whole so that when the sleepers drag the rails along the groove of the rails, the sleepers do not follow the movement or tip over. Thus, laying the rails becomes more convenient, smooth, and more efficient.