发明公开
EP2635595A2 OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
有权
荧光素酶OFF OPLOPHORUS，新COELENTERAZINSUBSTRATE和使用方法
- 专利标题： OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
- 专利标题（中）： 荧光素酶OFF OPLOPHORUS，新COELENTERAZINSUBSTRATE和使用方法
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申请号： EP11785534.6申请日： 2011-11-02
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公开(公告)号： EP2635595A2公开(公告)日： 2013-09-11
- 发明人: BINKOWSKI, Brock , ENCELL, Lance, P. , HALL, Mary , ROBERS, Matthew, B. , SLATER, Michael, R. , WOOD, Keith, V. , WOOD, Monika, G.
- 申请人： Promega Corporation
- 申请人地址： 2800 Woods Hollow Road Madison, WI 53711-5399 US
- 专利权人： Promega Corporation
- 当前专利权人： Promega Corporation
- 当前专利权人地址： 2800 Woods Hollow Road Madison, WI 53711-5399 US
- 代理机构： HOFFMANN EITLE
- 优先权： US409422P 20101102
- 国际公布： WO2012061530 20120510
- 主分类号： C07K14/435
- IPC分类号： C07K14/435
摘要：
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
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