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    MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS
    4.
    发明公开
    MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS 审中-公开
    MUTANTE PROTEASE-BIOSENSOREN MIT VERBESSERTEN ERKENNUNGSEIGENSCHAFTEN

    公开(公告)号：EP2776573A1

    公开(公告)日：2014-09-17

    申请号：EP12791055.2

    申请日：2012-11-12

    申请人： Promega Corporation

    发明人： BINKOWSKI, Brock ,  BUTLER, Braeden ,  ENCELL, Lance, P. ,  FAN, Frank ,  HOOK, Brad ,  OTTO, Paul ,  RISMA, Kimberly ,  VIDUGIRIS, Gediminas ,  WIGDAL, Susan ,  ZIMMERMAN, Kristopher

    IPC分类号： C12Q1/37 ,  C12Q1/66

    CPC分类号： C12Q1/66 ,  C12Q1/37

    摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly- permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly- permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

    摘要翻译： 编码生物传感器多肽的多核苷酸，其包含修饰的环状置换的热稳定性萤光素酶和将热稳定性萤光素酶的C末端部分连接到热稳定性萤光素酶的N末端部分的接头。 相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。 接头包含能够与细胞中的靶分子相互作用的传感器区域。 修饰的环状置换的热稳定性荧光素酶在目标分子存在下相对于亲本循环置换的热稳定性荧光素酶在生物传感器与靶分子相互作用后具有增强的响应。 或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在靶分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。