公开(公告)号：EP4306649A2

公开(公告)日：2024-01-17

申请号：EP23213374.4

申请日：2014-02-21

申请人： Promega Corporation

发明人： VALLEY, Michael, P. ,  CALI, James, J. ,  BINKOWSKI, Brock ,  EGGERS, Christopher, Todd ,  WOOD, Keith, V.

IPC分类号： C12Q1/66

摘要： Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.

公开(公告)号：EP3146066A1

公开(公告)日：2017-03-29

申请号：EP15730321.5

申请日：2015-05-26

申请人： Promega Corporation

发明人： KIRKLAND, Thomas ,  WOOD, Keith, V. ,  HALL, Mary ,  SCHWINN, Marie

IPC分类号： C12Q1/66 ,  C07D253/06

CPC分类号： C09K11/06 ,  A61K31/4985 ,  A61K31/53 ,  C07D253/075 ,  C07D487/04 ,  C09K2211/1007 ,  C09K2211/1044 ,  C12Q1/66

摘要： Disclosed herein are compositions and methods for stabilizing a luminogenic substrate such as coelenterazine or a functional analog thereof. The functional analog may be furimazine. The composition may include the luminogenic substrate, a thionucleoside, and an organic solvent, in which the thionucleoside is present in an amount effective to stabilize the luminogenic composition against decomposition. The method provided herein stabilizes the luminogenic substrate against decomposition by contacting the luminogenic substrate with an effective amount of the thionucleoside in the presence of the organic solvent. Also provided herein is a kit containing the composition.

摘要翻译： 本文公开了用于稳定发光底物如脑里脑炎或其功能类似物的组合物和方法。 功能类似物可以是呋嘧嗪。 组合物可以包括发光底物，硫核苷和有机溶剂，其中硫代核苷以有效稳定发光组合物以防分解的量存在。 本文提供的方法通过在有机溶剂存在下使发光底物与有效量的硫代核苷接触来稳定发光底物以防分解。 本文还提供了含有该组合物的试剂盒。

公开(公告)号：EP2004813B1

公开(公告)日：2015-06-03

申请号：EP07754666.1

申请日：2007-04-02

申请人： PROMEGA CORPORATION

发明人： BINKOWSKI, Brock ,  FAN, Frank ,  WIGDAL, Susan ,  WOOD, Keith, V. ,  WOOD, Monika, G.

IPC分类号： C12N9/02 ,  C12Q1/66 ,  G01N33/573 ,  C12N15/53

CPC分类号： C12N9/0069 ,  C12N9/0004 ,  C12Q1/66 ,  C12Y113/12007 ,  G01N33/84

公开(公告)号：EP1594962A2

公开(公告)日：2005-11-16

申请号：EP04707032.1

申请日：2004-01-30

申请人： PROMEGA CORPORATION

发明人： WOOD, Keith, V. ,  LOS, Georgyi, V. ,  BULLEIT, Robert, F. ,  KLAUBERT, Dieter ,  MCDOUGALL, Mark ,  ZIMPRICH, Chad

IPC分类号： C12N9/14 ,  C12Q1/34 ,  G01N33/58

CPC分类号： C12Q1/34 ,  C07C217/08 ,  C07C271/16 ,  C07D209/48 ,  C07D233/64 ,  C07D311/78 ,  C07D311/82 ,  C07D405/06 ,  C07D493/10 ,  C07D495/04 ,  C07F5/022 ,  C12N9/14 ,  C12N9/86 ,  C12Y308/01005 ,  G01N33/5005 ,  G01N33/58 ,  Y10S436/80

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变型水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：EP1155149A1

公开(公告)日：2001-11-21

申请号：EP00908725.5

申请日：2000-02-18

申请人： PROMEGA CORPORATION

发明人： SHULTZ, John, William ,  LEWIS, Martin, K. ,  LEIPPE, Donna ,  MANDREKAR, Michelle ,  KEPHART, Daniel ,  RHODES, Richard, B. ,  ANDREWS, Christine, Ann ,  HARTNETT, James, Robert ,  GU, Trent ,  OLSON, Ryan, J. ,  WOOD, Keith, V. ,  WELCH, Roy

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6823 ,  C12Q1/04 ,  C12Q1/66 ,  C12Q1/6827 ,  C12Q2535/131 ,  C12Q2521/319 ,  C12Q2565/301 ,  C12Q2565/1015 ,  C12Q2537/101 ,  C12Q2565/627

摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

公开(公告)号：EP2959010B1

公开(公告)日：2020-07-29

申请号：EP14709480.9

申请日：2014-02-21

申请人： Promega Corporation

发明人： VALLEY, Michael, P. ,  CALI, James, J. ,  BINKOWSKI, Brock ,  EGGERS, Christopher, Todd ,  WOOD, Keith, V.

IPC分类号： C12Q1/66

公开(公告)号：EP3146066B1

公开(公告)日：2018-03-14

申请号：EP15730321.5

申请日：2015-05-26

申请人： Promega Corporation

发明人： KIRKLAND, Thomas ,  WOOD, Keith, V. ,  HALL, Mary ,  SCHWINN, Marie

IPC分类号： C12Q1/66 ,  C07D253/06

CPC分类号： C09K11/06 ,  A61K31/4985 ,  A61K31/53 ,  C07D253/075 ,  C07D487/04 ,  C09K2211/1007 ,  C09K2211/1044 ,  C12Q1/66

摘要： Disclosed herein are compositions and methods for stabilizing a luminogenic substrate such as coelenterazine or a functional analog thereof. The functional analog may be furimazine. The composition may include the luminogenic substrate, a thionucleoside, and an organic solvent, in which the thionucleoside is present in an amount effective to stabilize the luminogenic composition against decomposition. The method provided herein stabilizes the luminogenic substrate against decomposition by contacting the luminogenic substrate with an effective amount of the thionucleoside in the presence of the organic solvent. Also provided herein is a kit containing the composition.

公开(公告)号：EP3191600A1

公开(公告)日：2017-07-19

申请号：EP15767679.2

申请日：2015-09-11

申请人： Promega Corporation

发明人： ENCELL, Lance, P. ,  HALL, Mary, P. ,  WOOD, Keith, V. ,  WOOD, Monika, G.

IPC分类号： C12Q1/66 ,  C12N9/02 ,  C12N15/53

CPC分类号： C12Q1/66 ,  C12N9/0069 ,  C12Y113/12007 ,  G01N21/6428 ,  G01N33/542 ,  G01N33/6803 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2333/90241

摘要： Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.

摘要翻译： 本文提供了编码经修饰的点击甲虫荧光素酶多肽的分离的多核苷酸，其具有增强的发光和较长波长的近红外信号。 本公开还涉及包括所述修饰的点击虫萤光素酶多肽和新颖虫荧光素衍生物的近红外生物发光系统，以及使用所述修饰的点击虫甲虫荧光素酶多肽和生物发光系统的方法。

公开(公告)号：EP1015601B1

公开(公告)日：2015-01-07

申请号：EP98948328.4

申请日：1998-09-18

申请人： PROMEGA CORPORATION

发明人： WOOD, Keith, V. ,  HALL, Mary, P.

IPC分类号： C12N15/53 ,  C12N9/02 ,  C12Q1/68

CPC分类号： C12N9/0069 ,  C12N15/10

公开(公告)号：EP1588143B1

公开(公告)日：2014-03-05

申请号：EP03800272.1

申请日：2003-12-23

申请人： PROMEGA CORPORATION

发明人： HAWKINS, Erika ,  CALI, James, J. ,  HO, Samuel, Kin, Sang ,  O'BRIEN, Martha ,  SOMBERG, Richard ,  BULLEIT, Robert, F. ,  WOOD, Keith, V.

IPC分类号： C12Q1/66 ,  C12Q1/68

CPC分类号： C12Q1/66