- 专利标题: Method for sorting cardiomyocytes
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申请号: US15127134申请日: 2015-03-20
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公开(公告)号: US10538740B2公开(公告)日: 2020-01-21
- 发明人: Yoshinori Yoshida , Hirohide Saito , Kenji Miki , Kei Endo , Seiya Takahashi
- 申请人: KYOTO UNIVERSITY
- 申请人地址: JP Kyoto
- 专利权人: Kyoto University
- 当前专利权人: Kyoto University
- 当前专利权人地址: JP Kyoto
- 代理机构: Myers Bigel, P.A.
- 优先权: JP2014-058926 20140320
- 国际申请: PCT/JP2015/058466 WO 20150320
- 国际公布: WO2015/141827 WO 20150924
- 主分类号: C12N5/077
- IPC分类号: C12N5/077 ; C12N5/00 ; C12N5/0735 ; A61K48/00
摘要:
An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.
公开/授权文献
- US20170369846A1 METHOD FOR SORTING CARDIOMYOCYTES 公开/授权日:2017-12-28
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