公开(公告)号：US10538740B2

公开(公告)日：2020-01-21

申请号：US15127134

申请日：2015-03-20

申请人： KYOTO UNIVERSITY

发明人： Yoshinori Yoshida ,  Hirohide Saito ,  Kenji Miki ,  Kei Endo ,  Seiya Takahashi

IPC分类号： C12N5/077 ,  C12N5/00 ,  C12N5/0735 ,  A61K48/00

摘要： An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.

公开(公告)号：US20180100203A1

公开(公告)日：2018-04-12

申请号：US15568209

申请日：2016-04-22

申请人： KYOTO UNIVERSITY

发明人： Yoshinori Yoshida ,  Hirohide Saito ,  Kenji Miki ,  Kei Endo ,  Seiya Takahashi

IPC分类号： C12Q1/6888

CPC分类号： C12Q1/6888 ,  C12N5/067 ,  C12N5/0676 ,  C12N5/069 ,  C12N15/113 ,  C12N15/67 ,  C12N2506/45 ,  C12Q1/68 ,  C12Q2600/178

摘要： An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.

公开(公告)号：US20170369846A1

公开(公告)日：2017-12-28

申请号：US15127134

申请日：2015-03-20

申请人： KYOTO UNIVERSITY

发明人： Yoshinori Yoshida ,  Hirohide Saito ,  Kenji Miki ,  Kei Endo ,  Seiya Takahashi

IPC分类号： C12N5/077 ,  C12N5/00 ,  C12N5/0735 ,  A61K48/00

摘要： An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.

公开(公告)号：US20200056248A1

公开(公告)日：2020-02-20

申请号：US16660333

申请日：2019-10-22

申请人： KYOTO UNIVERSITY

发明人： Yoshinori Yoshida ,  Hirohide Saito ,  Kenji Miki ,  Kei Endo ,  Seiya Takahashi

IPC分类号： C12Q1/6888 ,  C12N15/113 ,  C12Q1/68 ,  C12N15/67 ,  C12N5/071

摘要： An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.