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14 条记录 (耗时 0.018 秒)

    Translational control system using RNA-protein interaction motif
    1.
    发明授权
    Translational control system using RNA-protein interaction motif 有权
    使用RNA-蛋白质相互作用图案的翻译控制系统

    公开(公告)号：US09458466B2

    公开(公告)日：2016-10-04

    申请号：US14415009

    申请日：2013-07-16

    申请人： KYOTO UNIVERSITY

    发明人： Hirohide Saito ,  Kei Endo ,  Tan Inoue

    IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N15/67 ,  C07K14/32

    CPC分类号： C12N15/635 ,  C07K14/32 ,  C12N15/67

    摘要： A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5′UTR regulation structure comprising: (1) a cap structure at the 5′ terminus, (2) a spacer positioned on the 3′ side of the cap structure, and (3) one or more RNA motifs positioned on the 3′ side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3′ side of the 5′UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.

    摘要翻译： 提供了使用RNA-蛋白质相互作用基序的翻译控制方法。 该方法包括引入具有5'UTR调节结构的mRNA的步骤，其包括：（1）在5'末端的帽结构，（2）位于帽结构的3'侧的间隔区和（3 ）位于间隔区3'侧的一个或多个RNA基序，其包含RNA-蛋白质相互作用基序衍生的核苷酸序列或其变体; 和编码5'UTR调节结构3'侧的靶蛋白质基因的核苷酸序列在与RNA基序特异性结合的蛋白质存在下进入细胞，其中平移水平随着 间隔物减少，并且翻译水平随着RNA基序的数目增加而降低。

    TRANSLATIONAL CONTROL SYSTEM USING RNA-PROTEIN INTERACTION MOTIF
    5.
    发明申请
    TRANSLATIONAL CONTROL SYSTEM USING RNA-PROTEIN INTERACTION MOTIF 审中-公开

    公开(公告)号：US20190144873A1

    公开(公告)日：2019-05-16

    申请号：US16257676

    申请日：2019-01-25

    申请人： KYOTO UNIVERSITY

    发明人： Hirohide Saito ,  Kei Endo ,  Tan Inoue

    IPC分类号： C12N15/67

    摘要： A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5′UTR regulation structure comprising: (1) a cap structure at the 5′ terminus, (2) a spacer positioned on the 3′ side of the cap structure, and (3) one or more RNA motifs positioned on the 3′ side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3′ side of the 5′UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.

    Method for sorting cardiomyocytes
    7.
    发明授权
    Method for sorting cardiomyocytes 有权

    公开(公告)号：US10538740B2

    公开(公告)日：2020-01-21

    申请号：US15127134

    申请日：2015-03-20

    申请人： KYOTO UNIVERSITY

    发明人： Yoshinori Yoshida ,  Hirohide Saito ,  Kenji Miki ,  Kei Endo ,  Seiya Takahashi

    IPC分类号： C12N5/077 ,  C12N5/00 ,  C12N5/0735 ,  A61K48/00

    摘要： An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.

    METHOD FOR DETERMINING DESIRED CELL TYPE USING EXPRESSION OF miRNA AS INDICATOR
    9.
    发明申请
    METHOD FOR DETERMINING DESIRED CELL TYPE USING EXPRESSION OF miRNA AS INDICATOR 审中-公开
    使用miRNA作为指示剂的表达来确定所需细胞类型的方法

    公开(公告)号：US20170016077A1

    公开(公告)日：2017-01-19

    申请号：US15110503

    申请日：2015-01-09

    申请人： KYOTO UNIVERSITY

    发明人： Hirohide Saito ,  Kei Endo

    IPC分类号： C12Q1/68 ,  G01N21/64 ,  G01N15/14

    摘要： A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps:(1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.

    摘要翻译： 用于以高精度区分活体中的活细胞的方法。 一种利用miRNA作为指标区分所需细胞类型与包含两种或更多种类型细胞的细胞群的方法，其中该方法包括以下步骤：（1）可操作地引入包含标记基因的mRNA的步骤 与用作细胞组中的指示剂的miRNA的靶序列连接; 和（2）使用标记基因的翻译水平作为指标区分细胞类型的步骤。

    Method for extracting differentiated cells
    10.
    发明授权
    Method for extracting differentiated cells 有权

    公开(公告)号：US10604770B2

    公开(公告)日：2020-03-31

    申请号：US15326083

    申请日：2015-07-16

    申请人： KYOTO UNIVERSITY

    发明人： Hirohide Saito ,  Kei Endo ,  Shota Katayama ,  Callum Parr

    IPC分类号： C12N15/85 ,  C12N5/0793 ,  C12N5/074

    摘要： A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.