Invention Grant
- Patent Title: Method for the biotechnological production of flavone glycoside dihydrochalcones
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Application No.: US15322768Application Date: 2015-06-27
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Publication No.: US10538797B2Publication Date: 2020-01-21
- Inventor: Maren Thomsen , Jakob Ley , Egon Gross , Winfried Hinrichs , Uwe Bornscheuer
- Applicant: SYMRISE AG
- Applicant Address: DE Holzminden
- Assignee: SYMRISE AG
- Current Assignee: SYMRISE AG
- Current Assignee Address: DE Holzminden
- Agency: Polsinelli PC
- Priority: EP14175312 20140701
- International Application: PCT/EP2015/064626 WO 20150627
- International Announcement: WO2016/012198 WO 20160128
- Main IPC: C12P19/60
- IPC: C12P19/60 ; C12P17/06 ; A23L27/30

Abstract:
The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A′) according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A′) according to SEQ ID NO:4 has been cut out.
Public/Granted literature
- US20180216152A1 METHOD FOR THE BIOTECHNOLOGICAL PRODUCTION OF FLAVONE GLYCOSIDE DIHYDROCHALCONES Public/Granted day:2018-08-02
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