公开(公告)号：US10767198B2

公开(公告)日：2020-09-08

申请号：US15779214

申请日：2015-11-28

Applicant: SYMRISE AG

Inventor： Marco A. Fraatz ,  Holger Zorn ,  Johanna Rost ,  Michael Goldmann ,  Egon Gross ,  Jakob Ley ,  Katrin Geissler ,  Torsten Geissler ,  Michael Backes ,  Fabia Hentschel ,  Jens-Michael Hilmer

IPC: C12P7/24 ,  C07C45/27 ,  C12N9/02

Abstract: A method for producing branched aldehydes is proposed, the method comprising the following steps: (a) providing a culture of one or more fungi of the genus Conidiobolus and producing biomass containing branched carboxylic acids in free and/or bound form; (b) extracting the biomass from step (a) to produce a first intermediate containing free and/or bound carboxylic acids; (c) optionally chemically, enzymatically or microbially hydrolyzing the bound carboxylic acids from the first intermediate; (d) treating the first intermediate with a reducing agent of a chemical nature to convert the free and/or bound carboxylic acids into the corresponding alcohols and optionally separating one or more alcohols from interfering by-products and producing the chemically produced second intermediate containing these alcohols as a mixture or in enriched form; (e) treating the first intermediate with a reducing agent of a biological nature to convert the free and/or bound carboxylic acids into the corresponding aldehydes having the same number of carbon atoms compared to the free and/or bound carboxylic acids or into the corresponding aldehydes having a reduced number of carbon atoms by one compared to the free and/or bound carboxylic acids and producing the biologically produced second intermediate containing these aldehydes; (f) treating the chemically produced second intermediate with an oxidizing agent of a chemical nature to convert the free and/or bound alcohols into the corresponding aldehydes; and optionally (g) removing interfering by-components from the fractions obtainable after step(s) (d) and/or (e) and/or (f).

公开(公告)号：US09359622B2

公开(公告)日：2016-06-07

申请号：US13954957

申请日：2013-07-30

Applicant: Symrise AG

Inventor： Jens Michael Hilmer ,  Egon Gross ,  Gerhard Krammer ,  Jakob Peter Ley ,  Mechthild Gall ,  Uwe Bornscheuer ,  Maren Thomsen ,  Christin Peters ,  Patrick Jonczyk ,  Sascha Beutel ,  Thomas Scheper

IPC: C12P7/26 ,  C12N1/21 ,  C12N9/02 ,  C12N9/90 ,  C12N15/53 ,  C12N15/61 ,  C12N15/63 ,  C12R1/01 ,  C12R1/19 ,  C12R1/645 ,  C12R1/865 ,  C12R1/84

CPC classification number: C12P7/26 ,  C12N9/001 ,  C12N9/90 ,  C12R1/01 ,  C12R1/19 ,  C12R1/645 ,  C12R1/84 ,  C12R1/865 ,  C12Y103/01031 ,  C12Y505/01006 ,  Y02P20/52

Abstract: A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.

Abstract translation: 使用含有核酸部分（a）的转基因微生物来制造二氢查耳酮，特别是花旗素的二氢查耳酮的方法，其包含编码细菌查耳酮异构酶的基因和/或核酸部分（a'），或由编码细菌查耳酮异构酶的基因组成。 包含编码植物查耳酮异构酶的基因或核酸部分（b），其包含编码细菌酸酸酯还原酶的基因或由其组成，所述基因含有核酸部分（a），相应的转基因微生物，其包含 或由编码细菌查耳酮异构酶的基因和/或由编码植物查尔酮异构酶的基因组成或由编码植物查耳酮异构酶的核酸部分（a'）组成的核酸部分（a'），和/或核酸部分（b） 由编码细菌酸酯还原酶的基因和含有一个或多个相同或不同的这种载体的宿主细胞组成。

公开(公告)号：US10538797B2

公开(公告)日：2020-01-21

申请号：US15322768

申请日：2015-06-27

Applicant: SYMRISE AG

Inventor： Maren Thomsen ,  Jakob Ley ,  Egon Gross ,  Winfried Hinrichs ,  Uwe Bornscheuer

IPC: C12P19/60 ,  C12P17/06 ,  A23L27/30

Abstract: The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A′) according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A′) according to SEQ ID NO:4 has been cut out.

公开(公告)号：US20180216152A1

公开(公告)日：2018-08-02

申请号：US15322768

申请日：2015-06-27

Applicant: Symrise AG

Inventor： Maren Thomsen ,  Jakob Ley ,  Egon Gross ,  Winfried Hinrichs ,  Uwe Bornscheuer

IPC: C12P19/60 ,  C12P17/06 ,  A23L27/30

CPC classification number: C12P19/605 ,  A23L27/33 ,  A23V2002/00 ,  C12N9/001 ,  C12N9/90 ,  C12P17/06 ,  C12Y103/01031 ,  C12Y505/01006 ,  A23V2250/25

Abstract: The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A′) according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A′) according to SEQ ID NO:4 has been cut out.

公开(公告)号：US20140045233A1

公开(公告)日：2014-02-13

申请号：US13954957

申请日：2013-07-30

Applicant: Symrise AG

Inventor： Jens Michael Hilmer ,  Egon Gross ,  Gerhard Krammer ,  Jakob Peter Ley ,  Mechthild Gall ,  Uwe Bornscheuer ,  Maren Thomsen ,  Christin Peters ,  Patrick Jonczyk ,  Sascha Beutel ,  Thomas Scheper

IPC: C12P7/26 ,  C12R1/84 ,  C12R1/645 ,  C12R1/865 ,  C12R1/01 ,  C12R1/19

CPC classification number: C12P7/26 ,  C12N9/001 ,  C12N9/90 ,  C12R1/01 ,  C12R1/19 ,  C12R1/645 ,  C12R1/84 ,  C12R1/865 ,  C12Y103/01031 ,  C12Y505/01006 ,  Y02P20/52

Abstract: A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.

Abstract translation: 使用含有核酸部分（a）的转基因微生物来制造二氢查耳酮，特别是花旗素的二氢查耳酮的方法，其包含编码细菌查耳酮异构酶的基因和/或核酸部分（a'），或由编码细菌查耳酮异构酶的基因组成。 包含编码植物查耳酮异构酶的基因或核酸部分（b），其包含编码细菌酸酸酯还原酶的基因或由其组成，所述基因含有核酸部分（a），相应的转基因微生物，其包含 或由编码细菌查耳酮异构酶的基因和/或由编码植物查尔酮异构酶的基因组成或由编码植物查耳酮异构酶的核酸部分（a'）组成的核酸部分（a'），和/或核酸部分（b） 由编码细菌酸酯还原酶的基因和含有一个或多个相同或不同的这种载体的宿主细胞组成。