A memory device and a manufacturing method are provided. The memory device includes a plurality of memory cells stacked on a substrate. The memory cell includes two conductive patterns, a channel pillar, a gate pattern and a charge storage layer. The two conductive patterns are stacked on the substrate. The channel pillar extends between the two conductive patterns along a stacking direction of the two conductive patterns, and is electrically connected with the two conductive patterns. The gate pattern is disposed between the two conductive patterns and located at a sidewall of the channel pillar. The charge storage layer is disposed between the gate pattern and the channel pillar.