Invention Grant
- Patent Title: Method of immobilizing a cell on a support using compounds comprising a polyethylene glycol moiety
-
Application No.: US15186581Application Date: 2016-06-20
-
Publication No.: US10890585B2Publication Date: 2021-01-12
- Inventor: Hans-Peter Josel , Dieter Heindl , Thomas Froehlich , Stefanie Froehner
- Applicant: Roche Diagnostics Operations, Inc.
- Applicant Address: US IN Indianapolis
- Assignee: Roche Diagnostics Operations, Inc.
- Current Assignee: Roche Diagnostics Operations, Inc.
- Current Assignee Address: US IN Indianapolis
- Agency: Stinson LLP
- Priority: EP13006040 20131220
- Main IPC: C12N11/06
- IPC: C12N11/06 ; C12N11/08 ; G01N33/569 ; G01N33/50 ; G01N33/543 ; C12N5/00 ; C12N11/14

Abstract:
The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.
Public/Granted literature
- US20170363624A1 METHOD OF IMMOBILIZING A CELL ON A SUPPORT USING COMPOUNDS COMPRISING A POLYETHYLENE GLYCOL MOIETY Public/Granted day:2017-12-21
Information query