- 专利标题: Nanocapsule delivery system for ribonucleoproteins
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申请号: US16113963申请日: 2018-08-27
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公开(公告)号: US11013695B2公开(公告)日: 2021-05-25
- 发明人: Shaoqin Gong , Amr Abdeen , Krishanu Saha , Guojun Chen , Yuyuan Wang , Ruosen Xie
- 申请人: Wisconsin Alumni Research Foundation
- 申请人地址: US WI Madison
- 专利权人: Wisconsin Alumni Research Foundation
- 当前专利权人: Wisconsin Alumni Research Foundation
- 当前专利权人地址: US WI Madison
- 代理机构: Foley & Lardner LLP
- 主分类号: A61K9/51
- IPC分类号: A61K9/51 ; A61K48/00 ; A61K47/32 ; C12N15/11 ; A61K38/46 ; C12N15/113 ; A61K31/7105 ; A61K9/50 ; C12N9/22 ; C12N15/90
摘要:
Provided herein are nanocapsules comprising a single ribonucleoprotein (RNP) complex as a core and an biodegradable crosslinked polymer shell that encapsulates the core, wherein the RNP complex comprises a Cas9 polypeptide and a guide RNA, and the biodegradable crosslinked polymer shell comprises polymerized monomers of imidazolyl acryloyl monomers, bisacryloyl disulfide monomers (a biodegradable cross-linker), optionally PEG acryloyl monomers, and either cationic acryloyl monomers, anionic acryloyl monomers, or both cationic and anionic acryloyl monomers (optionally in combination with non-ionic acryloyl monomers) as defined herein. Also provided are methods of making the nanocapsules, kits containing the nanocapsules and methods of delivering the encapsulated RNP to cells.
公开/授权文献
- US20190099381A1 NANOCAPSULE DELIVERY SYSTEM FOR RIBONUCLEOPROTEINS 公开/授权日:2019-04-04