Invention Grant
- Patent Title: Transformed microorganism for producing PHA copolymer comprising 3HH monomer unit at high composition rate and method for producing PHA using same
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Application No.: US16927229Application Date: 2020-07-13
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Publication No.: US11453896B2Publication Date: 2022-09-27
- Inventor: Hisashi Arikawa , Shunsuke Sato
- Applicant: KANEKA CORPORATION
- Applicant Address: JP Osaka
- Assignee: KANEKA CORPORATION
- Current Assignee: KANEKA CORPORATION
- Current Assignee Address: JP Osaka
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- Priority: JPJP2018-005998 20180117
- Main IPC: C12P7/625
- IPC: C12P7/625 ; C12N9/10

Abstract:
The present application provides: a transformed microorganism for producing a PHA copolymer containing 3HH monomer unit at a higher composition ratio, specifically, a transformed microorganism comprising a PHA synthase gene capable of synthesizing a PHA copolymer containing 3HH monomer unit and a gene encoding a protein having (R)-specific enoyl-CoA hydratase activity, characterized in that, in the transformed microorganism, the expression of a gene encoding at least one β-ketothiolase enzyme having thiolysis activity for β-keto-(C6) acyl-CoA (i.e., β-ketohexanoyl-CoA) is inhibited, thereby losing or reducing the enzyme activity; and a method for producing a PHA copolymer containing 3HH monomer unit, comprising a step of culturing the transformed microorganism.
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