Invention Grant
- Patent Title: Suspension system for adeno associated virus production
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Application No.: US16798193Application Date: 2020-02-21
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Publication No.: US11608491B2Publication Date: 2023-03-21
- Inventor: Xin Yu , Xavier de Mollerat du Jeu , Chao Yan Liu , Jian Liu , Jonathan Zmuda
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Applicant Address: US CA Carlsbad
- Assignee: LIFE TECHNOLOGIES CORPORATION
- Current Assignee: LIFE TECHNOLOGIES CORPORATION
- Current Assignee Address: US CA Carlsbad
- Main IPC: C12N15/86
- IPC: C12N15/86 ; C12N5/10 ; C12N7/00 ; C07K14/005 ; C12N15/64 ; C12N1/06

Abstract:
The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.
Public/Granted literature
- US20200270583A1 SUSPENSION SYSTEM FOR ADENO ASSOCIATED VIRUS PROTECTION Public/Granted day:2020-08-27
Information query
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