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    SYSTEM AND METHOD FOR HIGH-YIELD TRANSIENT EXPRESSION IN MAMMALIAN CELLS
    2.
    发明申请
    SYSTEM AND METHOD FOR HIGH-YIELD TRANSIENT EXPRESSION IN MAMMALIAN CELLS 有权

    公开(公告)号：US20230130038A1

    公开(公告)日：2023-04-27

    申请号：US17977788

    申请日：2022-10-31

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Sanjay K. VASU ,  Chao Yan LIU ,  Jeffrey ROGERS ,  Maria Isabel CISNEROS ,  Jingqui LI ,  Henry C. CHIOU ,  Meredith Bauman JONES

    IPC分类号： C07K14/505 ,  C12N5/00 ,  C12N5/0793

    摘要： High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

    CELL ANALYSIS USING CHEMFET SENSOR ARRAY-BASED SYSTEMS
    3.
    发明申请
    CELL ANALYSIS USING CHEMFET SENSOR ARRAY-BASED SYSTEMS 有权

    公开(公告)号：US20230129295A1

    公开(公告)日：2023-04-27

    申请号：US18145688

    申请日：2022-12-22

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Wolfgang HINZ ,  John DONOHUE ,  Daniel BEACHAM

    IPC分类号： G01N27/414 ,  C09D189/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/407

    摘要： Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

    Methods, systems, and computer readable media for evaluating variant likelihood
    4.
    发明授权
    Methods, systems, and computer readable media for evaluating variant likelihood 有权

    公开(公告)号：US11636919B2

    公开(公告)日：2023-04-25

    申请号：US15974976

    申请日：2018-05-09

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Earl Hubbell ,  Sowmi Utiramerur

    IPC分类号： G16B20/20 ,  G16B30/00 ,  G16B40/00 ,  C12Q1/6874 ,  C12Q1/6869

    摘要： A method for evaluating variant likelihood includes: providing a plurality of template polynucleotide strands, sequencing primers, and polymerase in a plurality of defined spaces disposed on a sensor array; exposing the plurality of template polynucleotide strands, sequencing primers, and polymerase to a series of flows of nucleotide species according to a predetermined order; obtaining measured values corresponding to an ensemble of sequencing reads for at least some of the template polynucleotide strands in at least one of the defined spaces; and evaluating a likelihood that a variant sequence is present given the measured values corresponding to the ensemble of sequencing reads, the evaluating comprising: determining a measurement confidence value for each read in the ensemble of sequencing reads and modifying at least some model-predicted values using a first bias for forward strands and a second bias for reverse strands.

    Line Volume Calibration Systems And Methods
    7.
    发明申请
    Line Volume Calibration Systems And Methods 有权

    公开(公告)号：US20230119629A1

    公开(公告)日：2023-04-20

    申请号：US18069264

    申请日：2022-12-21

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Glen Davis

    IPC分类号： G01F25/17 ,  G01F15/00 ,  G01N29/22

    摘要： Provided are systems and methods for line volume calibration, and measurement of fluid samples delivered to an interrogation point. In various embodiments, a known fluid volume comprising a sample line fluid and a secondary fluid is delivered to a fluid boundary sensor. The fluid boundary sensor assists in determining the position of the boundaries between the various fluids, and the positions of these boundaries are used to determine the sample line fluid volume.

    APPARATUS AND METHODS FOR PERFORMING ELECTROCHEMICAL REACTIONS
    9.
    发明申请
    APPARATUS AND METHODS FOR PERFORMING ELECTROCHEMICAL REACTIONS 有权

    公开(公告)号：US20230101252A1

    公开(公告)日：2023-03-30

    申请号：US18077404

    申请日：2022-12-08

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： John NOBILE ,  George Thomas ROTH ,  Todd REARICK ,  Jonathan SCHULTZ ,  Jonathan M. ROTHBERG ,  David MARRAN

    IPC分类号： G01N27/30 ,  B01L3/00 ,  G01N27/414

    摘要： An apparatus includes a reaction vessels coupled to an electronic sensor for monitoring a reaction product in the reaction vessel; a fluidics system for sequentially delivering a plurality of reagents to the reaction vessel, the fluidics system including a plurality of reagent reservoirs in fluidic communication via a plurality of flow paths with a fluidics circuit and to a common passage in fluidic communication between the fluidics circuit and the reaction vessel, a solution reservoir in fluidic communication with the common passage via a branch passage connected with the common passage at a junction between the fluidics circuit and the reaction vessel; and an electrode in contact with a solution within the branch passage, the electrode being in electrical communication with the reaction vessel through fluid extending from the branch passage and through the common passage, the electronic sensor generating an output signal depending on a voltage of the electrode.