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    AMINE-CONTAINING TRANSFECTION REAGENTS AND METHODS FOR MAKING AND USING SAME
    6.
    发明申请
    AMINE-CONTAINING TRANSFECTION REAGENTS AND METHODS FOR MAKING AND USING SAME 有权
    含胺的转运试剂及其制备和使用方法

    公开(公告)号：US20150174260A1

    公开(公告)日：2015-06-25

    申请号：US14340395

    申请日：2014-07-24

    Applicant: Life Technologies Corporation

    Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt ,  Tao Wu

    IPC: A61K47/48 ,  C12N15/113 ,  A61K48/00 ,  C07C229/08

    CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

    Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

    Abstract translation: 本文提供了新颖的含胺转染化合物及其制备和使用方法。 化合物通常通过使伯胺与不饱和化合物反应而获得。 本文还提供了使用含胺转染化合物与附加化合物组合以包封生物活性剂如核酸制备的转染复合物。 还描述了使用转染复合物用于体内或体外递送生物活性剂的方法。 与本领域已知的类似转染化合物相比，本发明的转染复合物是非常有效的，从而允许相对低剂量的生物活性的有效调节。

    Serum-free suspension system for lentiviral production
    8.
    发明授权
    Serum-free suspension system for lentiviral production 有权

    公开(公告)号：US11414675B2

    公开(公告)日：2022-08-16

    申请号：US15721105

    申请日：2017-09-29

    Applicant: Life Technologies Corporation

    Inventor： Xin Yu ,  Xavier de Mollerat du Jeu

    IPC: C12N15/86 ,  C12N7/00

    Abstract: A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. Another method for lentiviral vector production comprises (a) culturing eukaryotic cells in a serum-free medium, (b) providing a lentiviral culture supplement to control cell growth, (c) transfecting the cells with a lentiviral vector using a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, and (d) providing a lentiviral production using a lentiviral production enhancer comprising sodium propionate, sodium butyrate capable of boosting lentiviral production.

    Compositions and methods for efficient delivery of molecules to cells
    9.
    发明授权
    Compositions and methods for efficient delivery of molecules to cells 有权

    公开(公告)号：US10195280B2

    公开(公告)日：2019-02-05

    申请号：US14800565

    申请日：2015-07-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Xavier de Mollerat du Jeu ,  Nektaria Andronikou

    IPC: A61K47/18 ,  A61K9/127 ,  A61K31/7105 ,  C12N15/88

    Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.

    Membrane-penetrating peptides to enhanced transfection and compositions and methods for using same
    10.
    发明授权
    Membrane-penetrating peptides to enhanced transfection and compositions and methods for using same 有权

    公开(公告)号：US12152248B2

    公开(公告)日：2024-11-26

    申请号：US17006629

    申请日：2020-08-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Xavier de Mollerat du Jeu

    IPC: C12N15/88 ,  A61K47/54 ,  A61K47/64 ,  C07K14/00

    Abstract: The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.

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