发明申请
- 专利标题: Method and device for integrated biomolecular analyses
- 专利标题(中): 一体化生物分子分析的方法和装置
-
申请号: US10486229申请日: 2002-08-07
-
公开(公告)号: US20050014146A1公开(公告)日: 2005-01-20
- 发明人: Nicolo Manaresi , Gianni Medoro , Luigi Altomare , Marco Tartagni , Roberto Guerrieri
- 申请人: Nicolo Manaresi , Gianni Medoro , Luigi Altomare , Marco Tartagni , Roberto Guerrieri
- 优先权: ITTO01A000801 20010807
- 国际申请: PCT/IT02/00524 WO 20020807
- 主分类号: B03C5/00
- IPC分类号: B03C5/00 ; G01N33/543 ; C12Q1/68 ; G01F1/64 ; G01N27/26 ; G01N33/50 ; G01N33/53
摘要:
A method whereby first biological entities are recognized by way of second biological entities able to bind to the first (or the first to the second), including the steps of binding first biological entities to a surface comprising an array of first electrodes selectively energizable and addressable at least in part, positioned facing at least one second electrode, bringing the second biological entities into contact with the first, these second biological entities and possibly the first being moved by means of dielectrophoretic cages generated between the electrodes, and sensing any binding activity between at least a portion of the first and of the second biological entities, preferably utilizing radiation at a first frequency to excite fluorophore groups bound to the second biological entities and detecting the emission of fluorescence at a second frequency by means of optical sensors integrated into the electrodes, the biological entities preferably being concentrated on the electrodes by the fusion of dielectrophoretic cages.