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公开(公告)号：US20050014146A1
公开(公告)日：2005-01-20
申请号：US10486229
申请日：2002-08-07
CPC分类号： B03C5/005 , G01N33/54373 , G01N2500/00
摘要： A method whereby first biological entities are recognized by way of second biological entities able to bind to the first (or the first to the second), including the steps of binding first biological entities to a surface comprising an array of first electrodes selectively energizable and addressable at least in part, positioned facing at least one second electrode, bringing the second biological entities into contact with the first, these second biological entities and possibly the first being moved by means of dielectrophoretic cages generated between the electrodes, and sensing any binding activity between at least a portion of the first and of the second biological entities, preferably utilizing radiation at a first frequency to excite fluorophore groups bound to the second biological entities and detecting the emission of fluorescence at a second frequency by means of optical sensors integrated into the electrodes, the biological entities preferably being concentrated on the electrodes by the fusion of dielectrophoretic cages.
摘要翻译： 一种方法，其中第一生物实体通过能够结合第一（或第一至第二）的第二生物实体识别，包括将第一生物实体结合到包括选择性可激发和可寻址的第一电极阵列的表面的步骤 至少部分地定位成面向至少一个第二电极，使得所述第二生物实体与所述第一，所述第二生物实体接触，并且可能的第一生物体可能通过在所述电极之间产生的介电电泳笼而移动，并且感测所述第二生物体之间的任何结合活性 所述第一和第二生物实体的至少一部分优选利用第一频率的辐射来激发与第二生物实体结合的荧光团，并且通过集成到电极中的光学传感器检测第二频率的荧光发射 生物实体优选集中在电极上 通过介电电泳笼的融合。
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2.发明授权Method and apparatus for high-throughput biological-activity screening of cells and/or compounds 有权用于细胞和/或化合物的高通量生物活性筛选的方法和装置公开(公告)号：US07699969B2
公开(公告)日：2010-04-20
申请号：US10476467
申请日：2002-05-02
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N33/54366 , G01N33/561
摘要： First entities consisting in cells or microorganisms (BIO) and second entities consisting in compounds or compound units, carried typically by microbeads (BEAD), are trapped selectively within closed movable potential cages (S1) by means of dielectrophoretic force generated by mutually opposed electrodes (M1, M2). The cages are set in relative motion so as to bring about the interaction of selected first and second entities, causing the cages containing them to fuse, whereupon results are obtained preferably by reinstating the original cages and/or observing previously empty adjacent cages. The procedure takes place in a device (DE) with two separate chambers (F, FL) connected one to the other by way of a narrow passage (D) and finished with respective selectively controllable inlets and outlets (I1, I2; O1, O2) through which a liquid or semi-liquid buffer (L) can be pumped in or out.
摘要翻译： 由细胞或微生物（BIO）组成的第一实体和通常由微珠（BEAD）携带的化合物或复合单元中的第二实体，通过由相互相对的电极产生的介电电泳力选择性地封闭在封闭的可移动电位笼（S1）内 M1，M2）。 笼子被设置为相对运动以便引起所选择的第一和第二实体的相互作用，导致包含它们的笼子融合，从而优选地通过恢复原始笼子和/或观察先前空的相邻笼子获得结果。 该程序在具有通过狭窄通道（D）彼此连接的两个分开的室（F，FL）的装置（DE）中进行，并且用相应的可选择性地控制的入口和出口（I1，I2; O1，O2 ）可以将液体或半液体缓冲液（L）泵入或流出。
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3.发明申请Method And Apparatus For Characterizing And Counting Particles, In Particular, Biological Particles 有权用于表征和计数颗粒的方法和装置，特别是生物颗粒公开(公告)号：US20090218223A1
公开(公告)日：2009-09-03
申请号：US12091438
申请日：2006-03-22
IPC分类号： G01N27/26
CPC分类号： G01N27/447 , C12M41/36
摘要： The present invention relates to a method and an apparatus for the characterization and/or the counting of particles by means of non uniform, time variable force fields and integrated optical or impedance meter sensors. The force fields can be of positive or negative dielectrophoresis, electrophoresis or electro-hydrodynamic motions, characterized by a set of stable equilibrium points for the particles (solid, liquid or gaseous); the same method is suitable for the manipulation of droplets (liquid particles) by exploiting effects known to the international scientific community with the name of Electro-wetting on dielectric. The aim of the present invention is to act on the control of the position of each particle which is present in the sample, for the purpose of displacing such particles in a deterministic or statistical way, in order to detect their presence with the integrated optical or impedance meter sensors and/or characterize their type, for the purpose of counting or manipulating them in an efficient way.
摘要翻译： 本发明涉及一种用于通过不均匀的时变力场和集成光学或阻抗计传感器表征和/或计数颗粒的方法和装置。 力场可以是正或负介电电泳，电泳或电流动力学运动，其特征在于用于颗粒（固体，液体或气体）的一组稳定的平衡点; 相同的方法适用于通过在电介质上以电润湿的名义利用国际科学界已知的影响来操纵液滴（液体颗粒）。 本发明的目的是作用于控制存在于样品中的每个颗粒的位置，以便以确定性或统计方式移动这些颗粒，以便用集成光学或 阻抗计传感器和/或表征其类型，以便以有效的方式计数或操纵它们。
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公开(公告)号：US20090218221A1
公开(公告)日：2009-09-03
申请号：US12091367
申请日：2006-10-23
申请人： Gianni Medoro , Nicolo Manaresi
发明人： Gianni Medoro , Nicolo Manaresi
IPC分类号： B01D57/02
摘要： The present invention relates to a method and apparatus for manipulation and/or control of the position of particles by means of fields of force of an electrical nature in electrically conductive solutions. The fields of force can be of (positive or negative) dielectrophoresis, electrophoresis, electrohydrodynamics or electrowetting on dielectric, characterized by a set of points of stable equilibrium for the particles. Each point of equilibrium can trap one or more particles within the attraction basin. Said forces dissipate by the Joule effect an amount of power proportional to the square of the voltages applied, causing in a short time the death of the biological particles contained in the specimen. According to the present invention, the dissipated power can be removed through at least one of the substrates in order to maintain the temperature in the liquid suspension constant or reduce it during the entire step of application of the forces. According to the present invention, the amount of heat to be extracted can be controlled by means of a temperature sensor internal to the microchamber or external thereto, which supplies information on the temperature of the system in order to establish a feedback control on the heat pump. In a second embodiment of the method, a flow constantly replaces the buffer, transporting by convection the heat outside the microchamber. Forming the subject of the present invention is likewise a method for minimizing the dissipated power given the same levels of performance by dividing the forces into classes, falling within one of which classes are the forces used for controlling particles in a static way whilst falling within a further class are the forces necessary for displacement of the particles. This can occur in a practical way by increasing the number of potentials that supply the electrodes of the device or else by appropriately modulating the amplitudes of the applied phases or by means of a timed management of the phases. Forming the subject of the present invention are likewise some practical implementations of the method that leads to an apparatus for manipulation of particles in conductive solutions. Said apparatus requires the use of a heat pump, which can be obtained by means of a Peltier-effect device or by means of the convective transport of the flow of heat absorbed by the substrate. Said convective flow uses a liquid or a gas. Forming the subject of the present invention is likewise an apparatus that exploits the gas law for reducing the temperature by varying the pressure of the gas having the function of performing the convective transport or by means of a change of phase from vapour to liquid or vice versa.
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公开(公告)号：US20080041723A1
公开(公告)日：2008-02-21
申请号：US11724697
申请日：2007-03-16
申请人： Nicolo Manaresi , Gianni Medoro
发明人： Nicolo Manaresi , Gianni Medoro
摘要： The invention pertains to a method and apparatus to separate and quantify particles using time-variable force fields. The force fields can be for dielectrophoresis (positive or negative), electrophoresis or electrohydrodynamic. In a first aspect of the method, the fields are translated and/or modified in space at a speed substantially comparable to the speed of translation of the fastest particles in the sample so that only these follow by changing position, while the slowest particles are not affected. According to the invention the translation and/or modification of the force field can also occur with varying speed, which is especially useful when this happens with periodic law on a field with spatial periodicity. In one aspect of the method, a force field with spatial periodicity is translated and/or modified in a first direction at high speed, for such a period of time as to cause a movement equal to the spatial period of the field, and at low speed in a second direction, opposite to the first one, for such a period of time as to cancel the overall movement of the field, causing the translation of the slowest particles in the second direction and no movement of the fastest particles. In an additional aspect of the method, the field is translated and/or modified in a first direction at high speed, for such a period of time as to cause a movement equal to the spatial period of the field minus a quantity corresponding to the period of the electrodes generating it, causing the translation of the fastest particles in the first direction and of the slowest particles in the opposite direction. In another aspect of the method, the quantity or size of the particles is determined by an indirect measurement of the speed of movement after varying the force field by means of a relationship between the speed of movement and the volume of the particles. This invention also pertains to an apparatus to produce appropriate field configurations that are necessary for the selective movement of particles
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6.发明申请公开(公告)号：US20090205963A1
公开(公告)日：2009-08-20
申请号：US11996068
申请日：2006-07-19
申请人： Gianni Medoro , Nicolo Manaresi
发明人： Gianni Medoro , Nicolo Manaresi
CPC分类号： G01N27/44756 , B03C5/026 , B03C2201/24 , G01N15/1031 , G01N15/1459
摘要： Method and apparatus for the manipulation and/or control of the position of particles by means of time-variable fields of force. The fields of force can be of dielectrophoresis (positive or negative), electrophoresis, electrohydrodynamic or electrowetting on dielectric, characterized by a set of stable points of equilibrium for the particles.
摘要翻译： 用于通过时变场力来操纵和/或控制颗粒位置的方法和装置。 力场可以是介电电泳（正或负），电泳，电动力学或电介质上的电润湿，其特征在于一组稳定的颗粒平衡点。
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公开(公告)号：US20120273356A9
公开(公告)日：2012-11-01
申请号：US11724697
申请日：2007-03-16
申请人： Nicolo Manaresi , Gianni Medoro
发明人： Nicolo Manaresi , Gianni Medoro
摘要： The invention pertains to a method and apparatus to separate and quantify particles using time-variable force fields. The force fields can be for dielectrophoresis (positive or negative), electrophoresis or electrohydrodynamic. In a first aspect of the method, the fields are translated and/or modified in space at a speed substantially comparable to the speed of translation of the fastest particles in the sample so that only these follow by changing position, while the slowest particles are not affected. According to the invention the translation and/or modification of the force field can also occur with varying speed, which is especially useful when this happens with periodic law on a field with spatial periodicity. In one aspect of the method, a force field with spatial periodicity is translated and/or modified in a first direction at high speed, for such a period of time as to cause a movement equal to the spatial period of the field, and at low speed in a second direction, opposite to the first one, for such a period of time as to cancel the overall movement of the field, causing the translation of the slowest particles in the second direction and no movement of the fastest particles. In an additional aspect of the method, the field is translated and/or modified in a first direction at high speed, for such a period of time as to cause a movement equal to the spatial period of the field minus a quantity corresponding to the period of the electrodes generating it, causing the translation of the fastest particles in the first direction and of the slowest particles in the opposite direction. In another aspect of the method, the quantity or size of the particles is determined by an indirect measurement of the speed of movement after varying the force field by means of a relationship between the speed of movement and the volume of the particles. This invention also pertains to an apparatus to produce appropriate field configurations that are necessary for the selective movement of particles
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8.发明授权Method and apparatus for characterizing and counting particles, in particular, biological particles 有权用于表征和计数颗粒，特别是生物颗粒的方法和装置公开(公告)号：US08679315B2
公开(公告)日：2014-03-25
申请号：US12091438
申请日：2006-03-22
IPC分类号： B01D57/02
CPC分类号： G01N27/447 , C12M41/36
摘要： The present invention relates to a method and an apparatus for the characterization and/or the counting of particles by means of non uniform, time variable force fields and integrated optical or impedance meter sensors. The force fields can be of positive or negative dielectrophoresis, electrophoresis or electro-hydrodynamic motions, characterized by a set of stable equilibrium points for the particles (solid, liquid or gaseous); the same method is suitable for the manipulation of droplets (liquid particles) by exploiting effects known to the international scientific community with the name of Electro-wetting on dielectric. The aim of the present invention is to act on the control of the position of each particle which is present in the sample, for the purpose of displacing such particles in a deterministic or statistical way, in order to detect their presence with the integrated optical or impedance meter sensors and/or characterize their type, for the purpose of counting or manipulating them in an efficient way.
摘要翻译： 本发明涉及一种用于通过不均匀的时变力场和集成的光学或阻抗计传感器表征和/或计数颗粒的方法和装置。 力场可以是正或负介电电泳，电泳或电流动力学运动，其特征在于用于颗粒（固体，液体或气体）的一组稳定的平衡点; 相同的方法适用于通过在电介质上以电润湿的名义利用国际科学界已知的影响来操纵液滴（液体颗粒）。 本发明的目的是作用于控制存在于样品中的每个颗粒的位置，以便以确定性或统计方式移动这些颗粒，以便用集成光学或 阻抗计传感器和/或表征其类型，以便以有效的方式计数或操纵它们。
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公开(公告)号：US09950322B2
公开(公告)日：2018-04-24
申请号：US13994158
申请日：2011-12-22
申请人： Gianni Medoro , Nicolò Manaresi
发明人： Gianni Medoro , Nicolò Manaresi
IPC分类号： B01L3/00 , B03C5/02 , G01N27/447
CPC分类号： B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/027 , B01L2200/04 , B01L2200/0652 , B01L2200/141 , B01L2300/023 , B01L2300/024 , B01L2300/0645 , B01L2300/0816 , B01L2300/0883 , B03C5/026 , G01N27/44791
摘要： A microfluidic device for isolating particles of at least one given type of a sample; the device is designed to be connected to an apparatus through a plurality of electrical connectors and comprises a system of microfluidic channels and a flash memory, which contains information on the structure (arrangement and geometry of the various components) of the system of microfluidic channels, a map of non-functioning parts of the device, the maximum number of uses and the maximum time of use of the device; the memory has portions allocated for storing the number of times and the time of use of the device.
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10.发明授权Method for the selection and/or the processing of particles, in particular cells 有权用于选择和/或处理颗粒，特别是细胞的方法公开(公告)号：US08926815B2
公开(公告)日：2015-01-06
申请号：US12297037
申请日：2007-04-13
申请人： Mélanie Abonnenc , Nicoló Manaresi , Gianni Medoro
发明人： Mélanie Abonnenc , Nicoló Manaresi , Gianni Medoro
摘要： Method for the selection or the processing of first particles sensitive to the application of an external stimulus including the step of producing, through the application of the external stimulus, the permeabilization of at least a selected first particle, consisting in the organization of the first particles through a first force field, to generate a second force field substantially placed in proximity of at least a selected first particle to be permeabilized.
摘要翻译： 用于选择或处理对施加外部刺激敏感的第一颗粒的方法，包括通过施加外部刺激产生至少所选择的第一颗粒的透化的步骤，所述至少一个选定的第一颗粒在组织第一颗粒 通过第一力场，以产生基本上放置在至少所选择的待透化的第一颗粒附近的第二力场。
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