公开(公告)号：US20050014146A1

公开(公告)日：2005-01-20

申请号：US10486229

申请日：2002-08-07

申请人： Nicolo Manaresi ,  Gianni Medoro ,  Luigi Altomare ,  Marco Tartagni ,  Roberto Guerrieri

发明人： Nicolo Manaresi ,  Gianni Medoro ,  Luigi Altomare ,  Marco Tartagni ,  Roberto Guerrieri

IPC分类号： B03C5/00 ,  G01N33/543 ,  C12Q1/68 ,  G01F1/64 ,  G01N27/26 ,  G01N33/50 ,  G01N33/53

CPC分类号： B03C5/005 ,  G01N33/54373 ,  G01N2500/00

摘要： A method whereby first biological entities are recognized by way of second biological entities able to bind to the first (or the first to the second), including the steps of binding first biological entities to a surface comprising an array of first electrodes selectively energizable and addressable at least in part, positioned facing at least one second electrode, bringing the second biological entities into contact with the first, these second biological entities and possibly the first being moved by means of dielectrophoretic cages generated between the electrodes, and sensing any binding activity between at least a portion of the first and of the second biological entities, preferably utilizing radiation at a first frequency to excite fluorophore groups bound to the second biological entities and detecting the emission of fluorescence at a second frequency by means of optical sensors integrated into the electrodes, the biological entities preferably being concentrated on the electrodes by the fusion of dielectrophoretic cages.

摘要翻译： 一种方法，其中第一生物实体通过能够结合第一（或第一至第二）的第二生物实体识别，包括将第一生物实体结合到包括选择性可激发和可寻址的第一电极阵列的表面的步骤 至少部分地定位成面向至少一个第二电极，使得所述第二生物实体与所述第一，所述第二生物实体接触，并且可能的第一生物体可能通过在所述电极之间产生的介电电泳笼而移动，并且感测所述第二生物体之间的任何结合活性 所述第一和第二生物实体的至少一部分优选利用第一频率的辐射来激发与第二生物实体结合的荧光团，并且通过集成到电极中的光学传感器检测第二频率的荧光发射 生物实体优选集中在电极上 通过介电电泳笼的融合。

公开(公告)号：US07699969B2

公开(公告)日：2010-04-20

申请号：US10476467

申请日：2002-05-02

申请人： Nicolò Manaresi ,  Gianni Medoro ,  Luigi Altomare ,  Marco Tartagni ,  Roberto Guerrieri

发明人： Nicolò Manaresi ,  Gianni Medoro ,  Luigi Altomare ,  Marco Tartagni ,  Roberto Guerrieri

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N33/54366 ,  G01N33/561

摘要： First entities consisting in cells or microorganisms (BIO) and second entities consisting in compounds or compound units, carried typically by microbeads (BEAD), are trapped selectively within closed movable potential cages (S1) by means of dielectrophoretic force generated by mutually opposed electrodes (M1, M2). The cages are set in relative motion so as to bring about the interaction of selected first and second entities, causing the cages containing them to fuse, whereupon results are obtained preferably by reinstating the original cages and/or observing previously empty adjacent cages. The procedure takes place in a device (DE) with two separate chambers (F, FL) connected one to the other by way of a narrow passage (D) and finished with respective selectively controllable inlets and outlets (I1, I2; O1, O2) through which a liquid or semi-liquid buffer (L) can be pumped in or out.

摘要翻译： 由细胞或微生物（BIO）组成的第一实体和通常由微珠（BEAD）携带的化合物或复合单元中的第二实体，通过由相互相对的电极产生的介电电泳力选择性地封闭在封闭的可移动电位笼（S1）内 M1，M2）。 笼子被设置为相对运动以便引起所选择的第一和第二实体的相互作用，导致包含它们的笼子融合，从而优选地通过恢复原始笼子和/或观察先前空的相邻笼子获得结果。 该程序在具有通过狭窄通道（D）彼此连接的两个分开的室（F，FL）的装置（DE）中进行，并且用相应的可选择性地控制的入口和出口（I1，I2; O1，O2 ）可以将液体或半液体缓冲液（L）泵入或流出。