发明申请
- 专利标题: Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site
- 专利标题(中): 使用肽切割位点从单个载体增强表达重组多肽的组合物和方法
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申请号: US10831304申请日: 2004-04-26
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公开(公告)号: US20050042721A1公开(公告)日: 2005-02-24
- 发明人: Jianmin Fang , Karin Jooss , Andrew Simmons , Jing-Jing Qian
- 申请人: Jianmin Fang , Karin Jooss , Andrew Simmons , Jing-Jing Qian
- 主分类号: C07H21/00
- IPC分类号: C07H21/00 ; C07H21/04 ; C07K1/00 ; C07K16/00 ; C07K16/18 ; C07K16/28 ; C12N20060101 ; C12N5/06 ; C12N15/00 ; C12N15/63 ; C12N15/64 ; C12N15/85 ; C12N15/86 ; C12N15/864 ; C12P21/02 ; C12P21/06
摘要:
Vector constructs for expression of two or more functional proteins or polypeptides under operative control of a single promoter and methods of making and using the same are described. The vectors comprise a self-processing cleavage site between each respective protein or polypeptide coding sequence. The vector constructs include the coding sequence for a self-processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from expressed protein(s) or polypeptide(s). The vector constructs find utility in methods for enhanced production of biologically active proteins and polypeptides in vitro and in vivo.