发明申请
- 专利标题: Multiphoton-excitation laser scanning microscope
- 专利标题(中): 多光子激发扫描显微镜
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申请号: US12148671申请日: 2008-04-21
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公开(公告)号: US20080266551A1公开(公告)日: 2008-10-30
- 发明人: Makoto Araki , Yasunari Matsukawa , Hiroshi Sasaki , Makio Ueno
- 申请人: Makoto Araki , Yasunari Matsukawa , Hiroshi Sasaki , Makio Ueno
- 申请人地址: JP Tokyo
- 专利权人: Olympus Corporation
- 当前专利权人: Olympus Corporation
- 当前专利权人地址: JP Tokyo
- 优先权: JP2007-117074 20070426
- 主分类号: G01N21/00
- IPC分类号: G01N21/00 ; G01J3/30
摘要:
A multiphoton-excitation laser scanning microscope capable of efficiently collecting fluorescence emitted from a specimen to acquire a brighter multiphoton-excitation fluorescence image is provided. This multiphoton-excitation laser scanning microscope includes a multiphoton-excitation laser light source for emitting ultrashort pulsed laser light, a light-scanning unit configured to scan a specimen with the ultrashort pulsed laser light emitted from the multiphoton-excitation laser light source in two dimensions, an objective lens configured to focus the ultrashort pulsed laser light scanned by the light-scanning unit on the specimen, a collector lens disposed opposite the objective lens, with the light-scanning unit disposed therebetween, to collect fluorescence emitted from the specimen, and a light detector configured to detect the fluorescence collected by the collector lens. The collector lens has a higher numerical aperture and a larger field number than the objective lens.
公开/授权文献
- US07889341B2 Multiphoton-excitation laser scanning microscope 公开/授权日:2011-02-15