发明申请
US20080299609A1 Uracil-DNA glycosylase of psychrobacter sp. HJ147 and use thereof
有权
精神病菌的尿嘧啶-DNA糖基化酶 HJ147及其用途
- 专利标题： Uracil-DNA glycosylase of psychrobacter sp. HJ147 and use thereof
- 专利标题（中）： 精神病菌的尿嘧啶-DNA糖基化酶 HJ147及其用途
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申请号： US11823123申请日： 2007-06-26
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公开(公告)号： US20080299609A1公开(公告)日： 2008-12-04
- 发明人: Suk-Tae Kwon , Mi-Sun Lee , Gun-A. Kim , Jung-Hyun Lee
- 申请人： Suk-Tae Kwon , Mi-Sun Lee , Gun-A. Kim , Jung-Hyun Lee
- 申请人地址： KR Suwon-si
- 专利权人： Sungkyunkwan University Foundation for Corporate Collaboration
- 当前专利权人： Sungkyunkwan University Foundation for Corporate Collaboration
- 当前专利权人地址： KR Suwon-si
- 优先权： KR10-2007-0023976 20070312
- 主分类号： C12P21/04
- IPC分类号： C12P21/04 ; C12N9/24 ; C12N15/11 ; C12N9/00 ; C12P19/34 ; C12N15/00 ; C12N1/20
摘要：
The present invention provides uracil-DNA glycosylase (UDG) gene originating from Psychrobacter sp. HJ147, and amino acid sequences deduced from the gene; expression and purification of Psp HJ147 UDG gene in Escherichia coli; and characterization of UDG obtained therefrom, and the use thereof in a polymerase chain reaction (PCR). The UDG according to the present invention has a specific activity of excising uracil bases in a uracil-containing DNA substrates at a low temperature, and is easily heat-inactivated. It thus can effectively eliminate cross contamination and carry-over contamination of PCR templates often occurring after a PCR process using dUTP. Therefore, it is useful for increasing preciseness (elimination of false positives), purity and amplification efficiency of PCR.
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