发明申请
US20090311749A1 Method for methanol independent induction from methanol inducible promoters in Pichia
失效
甲醇独立诱导甲基诱导启动子在毕赤酵母中的方法
- 专利标题: Method for methanol independent induction from methanol inducible promoters in Pichia
- 专利标题(中): 甲醇独立诱导甲基诱导启动子在毕赤酵母中的方法
-
申请号: US12521856申请日: 2008-01-25
-
公开(公告)号: US20090311749A1公开(公告)日: 2009-12-17
- 发明人: Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
- 申请人: Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
- 申请人地址: DK Bagsvaerd
- 专利权人: Novozymes A/S
- 当前专利权人: Novozymes A/S
- 当前专利权人地址: DK Bagsvaerd
- 优先权: DKPA200700122 20070126
- 国际申请: PCT/EP2008/050870 WO 20080125
- 主分类号: C12P21/00
- IPC分类号: C12P21/00
摘要:
The present invention relates to a method for producing a polypeptide in a methylotrophic yeast host cell, wherein expression of the polypeptide is controlled by a methanol inducible promoter, comprising: i) expression of a positive regulator from a non-native promoter, said positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
公开/授权文献
信息查询