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1.发明授权Method for methanol independent induction from methanol inducible promoters in Pichia 失效甲醇独立诱导甲基诱导启动子在毕赤酵母中的方法公开(公告)号：US08143023B2
公开(公告)日：2012-03-27
申请号：US12521856
申请日：2008-01-25
申请人： Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
发明人： Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
IPC分类号： C12P21/06
CPC分类号： C12P21/02 , C07K14/39 , C12N15/815
摘要： A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
摘要翻译： 描述了在甲基营养酵母宿主细胞中产生多肽的方法，其中多肽的表达由甲醇诱导型启动子控制，其包括：i）从非天然启动子表达阳性调节剂，阳性调节剂从 甲醇诱导型启动子，和ii）不加甲醇。
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2.发明申请METHOD FOR METHANOL INDEPENDENT INDUCTION FROM METHANOL INDUCIBLE PROMOTERS IN PICHIA 失效甲基葡萄糖独立诱导甲基淀粉诱导剂在PICHIA中的应用公开(公告)号：US20120142053A1
公开(公告)日：2012-06-07
申请号：US13396925
申请日：2012-02-15
申请人： Noriko Tsutsumi , Yuji Terui , Shinobu Takagi , XiangYu Kong
发明人： Noriko Tsutsumi , Yuji Terui , Shinobu Takagi , XiangYu Kong
CPC分类号： C12P21/02 , C07K14/39 , C12N15/815
摘要： A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
摘要翻译： 描述了在甲基营养酵母宿主细胞中产生多肽的方法，其中多肽的表达由甲醇诱导型启动子控制，其包括：i）从非天然启动子表达阳性调节剂，阳性调节剂从 甲醇诱导型启动子，和ii）不加甲醇。
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公开(公告)号：US08236528B2
公开(公告)日：2012-08-07
申请号：US13396925
申请日：2012-02-15
申请人： Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
发明人： Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
IPC分类号： C12P21/06
CPC分类号： C12P21/02 , C07K14/39 , C12N15/815
摘要： A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
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4.发明申请Method for methanol independent induction from methanol inducible promoters in Pichia 失效甲醇独立诱导甲基诱导启动子在毕赤酵母中的方法公开(公告)号：US20090311749A1
公开(公告)日：2009-12-17
申请号：US12521856
申请日：2008-01-25
申请人： Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
发明人： Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
IPC分类号： C12P21/00
CPC分类号： C12P21/02 , C07K14/39 , C12N15/815
摘要： The present invention relates to a method for producing a polypeptide in a methylotrophic yeast host cell, wherein expression of the polypeptide is controlled by a methanol inducible promoter, comprising: i) expression of a positive regulator from a non-native promoter, said positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
摘要翻译： 本发明涉及在甲基营养酵母宿主细胞中产生多肽的方法，其中多肽的表达由甲醇诱导型启动子控制，其包括：i）从非天然启动子，所述阳性调节子 激活甲醇诱导型启动子的转录，以及ii）不添加甲醇。
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公开(公告)号：US20100261259A1
公开(公告)日：2010-10-14
申请号：US12376372
申请日：2007-08-03
申请人： Tomoko Matsui , Pia Francke Johannesen , Shinobu Takagi , Thomas Agersten Poulsen , Noriko Tsutsumi , Zheng Liu , XiangYu Kong
发明人： Tomoko Matsui , Pia Francke Johannesen , Shinobu Takagi , Thomas Agersten Poulsen , Noriko Tsutsumi , Zheng Liu , XiangYu Kong
CPC分类号： C12N9/16 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.
摘要翻译： 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。
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公开(公告)号：US5929017A
公开(公告)日：1999-07-27
申请号：US809440
申请日：1997-04-24
申请人： Erik Gormsen , Naoko Ikegami , Masanobu Abo , Shinobu Takagi , Noriko Tsutsumi , Torben Halkier
发明人： Erik Gormsen , Naoko Ikegami , Masanobu Abo , Shinobu Takagi , Noriko Tsutsumi , Torben Halkier
CPC分类号： C11D3/38627 , C12N9/20 , Y10S435/912
摘要： A lipolytic enzyme with high activity at alkaline pH in the absence of Ca.sup.++ can be obtained from strains of filamentous fungi belonging to the genus Absidia. The lipolytic enzymes are effective for improving the effect of detergents towards fatty soiling.
摘要翻译： PCT No.PCT / DK95 / 00424 Sec。 371日期1997年04月24日 102（e）日期1997年4月24日PCT 1995年10月26日PCT PCT。 公开号WO96 / 13578 日期1996年5月9日在不含Ca ++的碱性pH下具有高活性的脂解酶可以从属于the属的丝状真菌菌株获得。 脂肪分解酶对于改善洗涤剂对脂肪污染的影响是有效的。
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公开(公告)号：US20110129874A1
公开(公告)日：2011-06-02
申请号：US13002631
申请日：2009-07-10
申请人： Noriko Tsutsumi , Shinobu Takagi
发明人： Noriko Tsutsumi , Shinobu Takagi
CPC分类号： C07K14/39 , C12N15/815
摘要： The present invention relates to promoter variants in the form of an isolated polynucleotide comprising: i) a nucleotide sequence consisting of the DAS promoter sequence from Pichia or a functional part thereof, wherein the said DAS promoter is contained in SEQ ID NO: 1; and ii) at least one additional UAS, wherein the said UAS is comprised in SEQ ID NO: 2.
摘要翻译： 本发明涉及分离多核苷酸形式的启动子变体，其包含：i）由毕赤氏酵母的DAS启动子序列或其功能部分组成的核苷酸序列，其中所述DAS启动子包含在SEQ ID NO：1中; 和ii）至少一种另外的UAS，其中所述UAS包含在SEQ ID NO：2中。
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公开(公告)号：US06509182B2
公开(公告)日：2003-01-21
申请号：US09892074
申请日：2001-06-26
申请人： Noriko Tsutsumi
发明人： Noriko Tsutsumi
IPC分类号： C12N920
CPC分类号： A21D8/042 , C11D3/38627 , C12N9/20
摘要： The present invention relates to lipolytic enzymes and DNA sequences encoding same. More specifically, the present invention relates to the lipolytic enzyme and DNA encoding same isolated from Acremonium berkeleyanum CBS 301.38 and analogues thereof.
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公开(公告)号：US07271139B2
公开(公告)日：2007-09-18
申请号：US11474151
申请日：2006-06-23
CPC分类号： C11D3/386 , A21D8/042 , C11D3/38627 , C12N9/20 , C12N15/1027
摘要： The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).
摘要翻译： 本发明人已经分离出与丝状芽孢杆菌脂肪酶基因具有高同源性的新型脂肪分解酶基因，因此非常适合用于基因改组。 因此，本发明提供了一种通过编码脂肪分解酶的两个或更多个同源基因的家族洗牌来产生脂肪分解酶的遗传多样性的方法。 DNA混洗技术用于创建洗牌基因文库，并在合适的表达系统中表达，并筛选表达的蛋白质以进行脂肪分解酶活性。 本发明还提供了包含编码脂肪分解酶和脂肪分解酶（具有脂肪分解酶活性的多肽）的核苷酸序列的多核苷酸。
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公开(公告)号：US20070010418A1
公开(公告)日：2007-01-11
申请号：US11474151
申请日：2006-06-23
申请人： Noriko Tsutsumi , Jesper Vind , Shamkant Anant
发明人： Noriko Tsutsumi , Jesper Vind , Shamkant Anant
CPC分类号： C11D3/386 , A21D8/042 , C11D3/38627 , C12N9/20 , C12N15/1027
摘要： The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).
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