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39 条记录 (耗时 0.025 秒)

    Expression of Genes from Gram Negative Bacteria in Fungi
    5.
    发明申请
    Expression of Genes from Gram Negative Bacteria in Fungi 审中-公开
    革兰氏阴性菌在真菌中的表达

    公开(公告)号：US20100261259A1

    公开(公告)日：2010-10-14

    申请号：US12376372

    申请日：2007-08-03

    申请人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    发明人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    IPC分类号： C12N1/19 ,  C12N1/15 ,  C07H21/04

    CPC分类号： C12N9/16 ,  C07K2319/02 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.

    摘要翻译： 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。

    Pichia Pastoris Das Promoter Variants
    6.
    发明申请
    Pichia Pastoris Das Promoter Variants 审中-公开
    巴斯德毕赤酵母Das启动子变体

    公开(公告)号：US20110129874A1

    公开(公告)日：2011-06-02

    申请号：US13002631

    申请日：2009-07-10

    申请人： Noriko Tsutsumi ,  Shinobu Takagi

    发明人： Noriko Tsutsumi ,  Shinobu Takagi

    IPC分类号： C12P21/02 ,  C07H21/04 ,  C12N15/63 ,  C12N1/19

    CPC分类号： C07K14/39 ,  C12N15/815

    摘要： The present invention relates to promoter variants in the form of an isolated polynucleotide comprising: i) a nucleotide sequence consisting of the DAS promoter sequence from Pichia or a functional part thereof, wherein the said DAS promoter is contained in SEQ ID NO: 1; and ii) at least one additional UAS, wherein the said UAS is comprised in SEQ ID NO: 2.

    摘要翻译： 本发明涉及分离多核苷酸形式的启动子变体，其包含：i）由毕赤氏酵母的DAS启动子序列或其功能部分组成的核苷酸序列，其中所述DAS启动子包含在SEQ ID NO：1中; 和ii）至少一种另外的UAS，其中所述UAS包含在SEQ ID NO：2中。

    Method for producing RFeB-based magnet
    8.
    发明授权
    Method for producing RFeB-based magnet 有权
    制造基于RFeB的磁体的方法

    公开(公告)号：US09368277B2

    公开(公告)日：2016-06-14

    申请号：US14492444

    申请日：2014-09-22

    申请人： Shinobu Takagi

    发明人： Shinobu Takagi

    IPC分类号： B05D5/00 ,  H01F41/16 ,  H01F1/053 ,  H01F1/055 ,  H01F41/02

    CPC分类号： H01F41/16 ,  B05D5/00 ,  H01F1/0536 ,  H01F1/0551 ,  H01F41/0293

    摘要： Provided is a method for producing an RFeB-based magnet, the method including: disposing a nozzle so as to be opposed to an attachment surface of a base material that is a sintered magnet or hot-plastic worked magnet composed of an RFeB-based magnet containing a light rare earth element RL that is at least one element selected from the group consisting of Nd and Pr, Fe, and B; ejecting a mixture, from the nozzle, obtained by mixing an organic solvent and an RH-containing powder containing a heavy rare earth element RH that is at least one element selected from the group consisting of Dy, Tb and Ho so as to attach the mixture to the attachment surface; and heating the base material together with the mixture.

    摘要翻译： 本发明提供一种RFeB系磁体的制造方法，其特征在于，包括：将喷嘴配置成与由烧结磁体构成的基材的配合面或由RFeB系磁铁构成的热塑性加工磁铁 含有选自Nd和Pr，Fe和B中的至少一种元素的轻稀土元素RL; 喷射通过混合有机溶剂和含有选自Dy，Tb和Ho中的至少一种元素的重稀土元素RH的含RH粉末得到的混合物，以便将混合物 到附件表面; 并与混合物一起加热基材。

    Lipoxygenase
    10.
    发明授权
    Lipoxygenase 失效
    脂氧合酶

    公开(公告)号：US07456001B2

    公开(公告)日：2008-11-25

    申请号：US10362776

    申请日：2001-09-05

    申请人： Soren Christensen ,  Akiko Sugio ,  Shinobu Takagi ,  Lars Ostergaard ,  Ernst Oliw

    发明人： Soren Christensen ,  Akiko Sugio ,  Shinobu Takagi ,  Lars Ostergaard ,  Ernst Oliw

    IPC分类号： C12N9/02 ,  C12N15/00 ,  C12N1/20 ,  C12P21/04 ,  A21D10/00 ,  C11D3/02 ,  C07H21/04 ,  C12Q1/00 ,  C12Q1/68

    CPC分类号： C12N9/0069

    摘要： The invention provides sequence information of a microbial protein having lipoxy-genase activity and a method of producing the protein by recombinant DNA technology. More specifically, the inventors have isolated a gene encoding a lipoxygenase from Gaeu-mannomyces graminis, cloned it into an E. coli strain and sequenced it. A comparison shows less than 25% identity to known lipoxygenase sequences, the closest being human 15S li-poxygenase. The inventors have expressed the lipoxygenase recombinantly and found that the recombinant lipoxygenase is glycosylated.

    摘要翻译： 本发明提供具有脂氧合酶活性的微生物蛋白质的序列信息和通过重组DNA技术生产该蛋白质的方法。 更具体地说，本发明人已经分离出来自禾谷镰刀菌的编码脂氧合酶的基因，将其克隆到大肠杆菌菌株中并对其进行测序。 比较显示与已知的脂氧合酶序列具有小于25％的同一性，最接近的是人15S li-加氧酶。 本发明人重组表达了脂氧合酶，发现重组脂氧合酶被糖基化。