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    Lipolytic enzymes
    1.
    发明授权
    Lipolytic enzymes 失效

    公开(公告)号：US06509182B2

    公开(公告)日：2003-01-21

    申请号：US09892074

    申请日：2001-06-26

    申请人： Noriko Tsutsumi

    发明人： Noriko Tsutsumi

    IPC分类号： C12N920

    CPC分类号： A21D8/042 ,  C11D3/38627 ,  C12N9/20

    摘要： The present invention relates to lipolytic enzymes and DNA sequences encoding same. More specifically, the present invention relates to the lipolytic enzyme and DNA encoding same isolated from Acremonium berkeleyanum CBS 301.38 and analogues thereof.

    Expression of Genes from Gram Negative Bacteria in Fungi
    3.
    发明申请
    Expression of Genes from Gram Negative Bacteria in Fungi 审中-公开
    革兰氏阴性菌在真菌中的表达

    公开(公告)号：US20100261259A1

    公开(公告)日：2010-10-14

    申请号：US12376372

    申请日：2007-08-03

    申请人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    发明人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    IPC分类号： C12N1/19 ,  C12N1/15 ,  C07H21/04

    CPC分类号： C12N9/16 ,  C07K2319/02 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.

    摘要翻译： 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。

    Lipolytic enzyme genes
    4.
    发明授权
    Lipolytic enzyme genes 有权
    脂解酶基因

    公开(公告)号：US07271139B2

    公开(公告)日：2007-09-18

    申请号：US11474151

    申请日：2006-06-23

    申请人： Noriko Tsutsumi ,  Jesper Vind ,  Shamkant Anant Patkar

    发明人： Noriko Tsutsumi ,  Jesper Vind ,  Shamkant Anant Patkar

    IPC分类号： A21D2/00 ,  C12N9/20

    CPC分类号： C11D3/386 ,  A21D8/042 ,  C11D3/38627 ,  C12N9/20 ,  C12N15/1027

    摘要： The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).

    摘要翻译： 本发明人已经分离出与丝状芽孢杆菌脂肪酶基因具有高同源性的新型脂肪分解酶基因，因此非常适合用于基因改组。 因此，本发明提供了一种通过编码脂肪分解酶的两个或更多个同源基因的家族洗牌来产生脂肪分解酶的遗传多样性的方法。 DNA混洗技术用于创建洗牌基因文库，并在合适的表达系统中表达，并筛选表达的蛋白质以进行脂肪分解酶活性。 本发明还提供了包含编码脂肪分解酶和脂肪分解酶（具有脂肪分解酶活性的多肽）的核苷酸序列的多核苷酸。

    Lipolytic enzyme genes
    5.
    发明申请
    Lipolytic enzyme genes 有权

    公开(公告)号：US20070010418A1

    公开(公告)日：2007-01-11

    申请号：US11474151

    申请日：2006-06-23

    申请人： Noriko Tsutsumi ,  Jesper Vind ,  Shamkant Anant

    发明人： Noriko Tsutsumi ,  Jesper Vind ,  Shamkant Anant

    IPC分类号： C11D3/386 ,  C07H21/04 ,  C12P21/06 ,  C12N15/74 ,  C12N1/21 ,  C12N9/20

    CPC分类号： C11D3/386 ,  A21D8/042 ,  C11D3/38627 ,  C12N9/20 ,  C12N15/1027

    摘要： The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).

    Lipolytic enzymes
    6.
    发明授权
    Lipolytic enzymes 失效

    公开(公告)号：US06511837B2

    公开(公告)日：2003-01-28

    申请号：US09891735

    申请日：2001-06-26

    申请人： Noriko Tsutsumi

    发明人： Noriko Tsutsumi

    IPC分类号： C12N920

    CPC分类号： C12N9/20 ,  A21D8/042 ,  C11D3/38627

    摘要： The present invention relates to lipolytic enzymes and DNA sequences encoding same. More specifically, the present invention relates to the lipolytic enzyme and DNA encoding same isolated from Fusarium culmorum CBS 513.94 and analogues thereof.

    Starch conversion process
    7.
    发明授权
    Starch conversion process 有权
    淀粉转化过程

    公开(公告)号：US06448049B1

    公开(公告)日：2002-09-10

    申请号：US09544123

    申请日：2000-04-06

    申请人： Noriko Tsutsumi ,  Henrik Bisgård-Frantzen ,  Allan Svendsen

    发明人： Noriko Tsutsumi ,  Henrik Bisgård-Frantzen ,  Allan Svendsen

    IPC分类号： C12N944

    CPC分类号： C12N9/246 ,  C12N9/2451 ,  C12P19/16 ,  C12Y302/01001 ,  C12Y302/01068

    摘要： The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.

    摘要翻译： 本发明涉及一种淀粉转化方法，其包括脱支步骤，其中使用主要工艺条件的活性的异淀粉酶用于脱淀淀粉和使用热稳定的异戊酰化酶进行淀粉转化。 本发明还涉及从Rhodothermus菌株获得的分离的异淀粉酶，以及克隆编码来自Rhodothermus或Sulfolobus菌株的异戊酰化酶的DNA序列，包含所述DNA序列的表达载体，包含该表达载体的宿主细胞，最后到 生产所述异戊酰化酶的方法。