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发明申请
US20100173298A1 HCV GENE 审中-公开
HCV基因

HCV GENE
摘要：
The present invention provides a hepatitis C virus gene, a replicon RNA derived from the gene, a replicon-replicating cell into which the replicon RNA is introduced, and a method for screening a drug using the replicon-replicating cell. By introducing a replicon RNA comprising (A) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 5; (B) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 7; (C) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 6; (D) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 8; or (E) a polynucleotide having a nucleic acid sequence having a homology of not less than 90% with the nucleic acid sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7; or a replicon RNA of the genotype 1b comprising nucleotides coding for the 1804th amino acid leucine and the 1966th amino acid lysine in the amino acid sequence of a hepatitis C virus polyprotein and a polynucleotide coding for an NS4B protein, into a cell, the replicon-replicating cell can be prepared. By using the replicon-replicating cell, the screening for the drug can be carried out.
摘要（中）：

本发明提供丙型肝炎病毒基因，来源于该基因的复制子RNA，引入复制子RNA的复制子复制细胞和使用复制子复制细胞筛选药物的方法。 通过引入包含（A）具有SEQ ID NO：5所示核酸序列的多核苷酸的复制子RNA; （B）具有SEQ ID NO：7所示核酸序列的多核苷酸; （C）编码具有SEQ ID NO：6所示氨基酸序列的多肽的多核苷酸; （D）编码具有SEQ ID NO：8所示氨基酸序列的多肽的多核苷酸; 或（E）具有与SEQ ID NO：5或SEQ ID NO：7所示核酸序列同源性不低于90％的核酸序列的多核苷酸; 或基因型1b的复制子RNA，其包含编码丙型肝炎病毒多蛋白的氨基酸序列中的第1804位氨基酸亮氨酸和第1966位氨基酸赖氨酸的核苷酸和编码NS4B蛋白的多核苷酸的复制子RNA， 可以制备复制细胞。 通过使用复制子复制细胞，可以进行药物的筛选。

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