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公开(公告)号：US20100173298A1
公开(公告)日：2010-07-08
申请号：US12597692
申请日：2008-04-28
申请人： Kenichi Mori , Noboru Maki , Hiromi Fukai , Chiharu Ohue
发明人： Kenichi Mori , Noboru Maki , Hiromi Fukai , Chiharu Ohue
CPC分类号： G01N33/5008 , C07K14/005 , C12N15/86 , C12N2770/24222 , C12N2770/24243 , C12Q1/707 , G01N33/5767 , G01N2333/186 , G01N2500/00
摘要： The present invention provides a hepatitis C virus gene, a replicon RNA derived from the gene, a replicon-replicating cell into which the replicon RNA is introduced, and a method for screening a drug using the replicon-replicating cell. By introducing a replicon RNA comprising (A) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 5; (B) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 7; (C) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 6; (D) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 8; or (E) a polynucleotide having a nucleic acid sequence having a homology of not less than 90% with the nucleic acid sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7; or a replicon RNA of the genotype 1b comprising nucleotides coding for the 1804th amino acid leucine and the 1966th amino acid lysine in the amino acid sequence of a hepatitis C virus polyprotein and a polynucleotide coding for an NS4B protein, into a cell, the replicon-replicating cell can be prepared. By using the replicon-replicating cell, the screening for the drug can be carried out.
摘要翻译： 本发明提供丙型肝炎病毒基因，来源于该基因的复制子RNA，引入复制子RNA的复制子复制细胞和使用复制子复制细胞筛选药物的方法。 通过引入包含（A）具有SEQ ID NO：5所示核酸序列的多核苷酸的复制子RNA; （B）具有SEQ ID NO：7所示核酸序列的多核苷酸; （C）编码具有SEQ ID NO：6所示氨基酸序列的多肽的多核苷酸; （D）编码具有SEQ ID NO：8所示氨基酸序列的多肽的多核苷酸; 或（E）具有与SEQ ID NO：5或SEQ ID NO：7所示核酸序列同源性不低于90％的核酸序列的多核苷酸; 或基因型1b的复制子RNA，其包含编码丙型肝炎病毒多蛋白的氨基酸序列中的第1804位氨基酸亮氨酸和第1966位氨基酸赖氨酸的核苷酸和编码NS4B蛋白的多核苷酸的复制子RNA， 可以制备复制细胞。 通过使用复制子复制细胞，可以进行药物的筛选。
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公开(公告)号：US20110289610A1
公开(公告)日：2011-11-24
申请号：US13054356
申请日：2009-07-15
申请人： Noboru Maki , Kenichi Mori , Hiromi Fukai
发明人： Noboru Maki , Kenichi Mori , Hiromi Fukai
CPC分类号： C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222 , C12N2770/24243
摘要： An HCV/GBV-B chimeric virus which maintains the replication function of HCV and is capable of infecting tamarin is disclosed in order to construct an HCV animal model which can be used as a development or evaluation system for therapeutic agents for HCV. The HCV/GBV-B chimeric RNA comprises an RNA of hepatitis C virus and an RNA of GB virus-B, wherein the RNA of hepatitis C virus comprises an RNA encoding leucine at the 1804th position and lysine at the 1966th position in the amino acid sequence of the polyprotein of hepatitis C virus.
摘要翻译： 公开了保持HCV的复制功能并能够感染t am素的HCV / GBV-B嵌合病毒，以构建可用作HCV治疗剂的开发或评估系统的HCV动物模型。 HCV / GBV-B嵌合RNA包含丙型肝炎病毒的RNA和GB病毒B的RNA，其中丙型肝炎病毒的RNA包含编码第1804位亮氨酸的RNA，氨基酸中第1966位的赖氨酸 丙型肝炎病毒多蛋白序列。
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公开(公告)号：US20130189779A1
公开(公告)日：2013-07-25
申请号：US13823422
申请日：2011-10-07
申请人： Kenichi Mori , Noboru Maki , Hiromi Fukai
发明人： Kenichi Mori , Noboru Maki , Hiromi Fukai
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , C07K14/005 , C12N2770/24222
摘要： Disclosed are an HCV gene having higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b, an RNA replicon having this gene, a cell infected with this RNA replicon, which cell allows replication of HCV, and an HCV particle. The hepatitis C virus gene encodes an amino acid sequence wherein the 979th amino acid is threonine; the 1804th amino acid is leucine; and the 1966th amino acid is lysine. An HCV gene which can propagate in vitro and has higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b was provided.
摘要翻译： 公开了具有比基因型1b的已知HCV基因，具有该基因的RNA复制子，感染了该RNA复制子的细胞，该细胞允许HCV复制和HCV颗粒更高的复制效率和更高的再感染效率的HCV基因。 丙型肝炎病毒基因编码氨基酸序列，其中第979位氨基酸是苏氨酸; 第1804位氨基酸为亮氨酸; 而第1966位氨基酸是赖氨酸。 提供了可以在体外繁殖并且具有比已知的基因型1b的HCV基因更高的复制效率和更高的再感染效率的HCV基因。
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公开(公告)号：US08981073B2
公开(公告)日：2015-03-17
申请号：US13823422
申请日：2011-10-07
申请人： Kenichi Mori , Noboru Maki , Hiromi Fukai
发明人： Kenichi Mori , Noboru Maki , Hiromi Fukai
IPC分类号： C07H21/04 , A01N63/02 , C12N7/00 , C07H21/02 , C07K14/005
CPC分类号： C07H21/02 , C07K14/005 , C12N2770/24222
摘要： Disclosed are an HCV gene having higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b, an RNA replicon having this gene, a cell infected with this RNA replicon, which cell allows replication of HCV, and an HCV particle. The hepatitis C virus gene encodes an amino acid sequence wherein the 979th amino acid is threonine; the 1804th amino acid is leucine; and the 1966th amino acid is lysine. An HCV gene which can propagate in vitro and has higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b was provided.
摘要翻译： 公开了具有比基因型1b的已知HCV基因，具有该基因的RNA复制子，感染了该RNA复制子的细胞，该细胞允许HCV复制和HCV颗粒更高的复制效率和更高的再感染效率的HCV基因。 丙型肝炎病毒基因编码氨基酸序列，其中第979位氨基酸是苏氨酸; 第1804位氨基酸为亮氨酸; 而第1966位氨基酸是赖氨酸。 提供了可以在体外繁殖并且具有比已知的基因型1b的HCV基因更高的复制效率和更高的再感染效率的HCV基因。
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公开(公告)号：US08741607B2
公开(公告)日：2014-06-03
申请号：US13054356
申请日：2009-07-15
申请人： Noboru Maki , Kenichi Mori , Hiromi Fukai
发明人： Noboru Maki , Kenichi Mori , Hiromi Fukai
CPC分类号： C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222 , C12N2770/24243
摘要： An HCV/GBV-B chimeric virus which maintains the replication function of HCV and is capable of infecting tamarin is disclosed in order to construct an HCV animal model which can be used as a development or evaluation system for therapeutic agents for HCV. The HCV/GBV-B chimeric RNA comprises an RNA of hepatitis C virus and an RNA of GB virus-B, wherein the RNA of hepatitis C virus comprises an RNA encoding leucine at the 1804th position and lysine at the 1966th position in the amino acid sequence of the polyprotein of hepatitis C virus.
摘要翻译： 公开了保持HCV的复制功能并能够感染t am素的HCV / GBV-B嵌合病毒，以构建可用作HCV治疗剂的开发或评估系统的HCV动物模型。 HCV / GBV-B嵌合RNA包含丙型肝炎病毒的RNA和GB病毒B的RNA，其中丙型肝炎病毒的RNA包含编码第1804位亮氨酸的RNA，氨基酸中第1966位的赖氨酸 丙型肝炎病毒多蛋白序列。
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