发明申请
- 专利标题: Variants of An Alpha-Amylase with Improved Production Levels in Fermentation Processes
- 专利标题(中): 在发酵过程中提高生产水平的α-淀粉酶的变体
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申请号: US12601759申请日: 2008-05-29
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公开(公告)号: US20110027252A1公开(公告)日: 2011-02-03
- 发明人: Wolfgang Aehle , Neelam S. Amin
- 申请人: Wolfgang Aehle , Neelam S. Amin
- 国际申请: PCT/US08/06787 WO 20080529
- 主分类号: A01N63/02
- IPC分类号: A01N63/02 ; C07H21/04 ; C12N1/21 ; C12N15/63 ; C12N9/26 ; C12N9/98 ; C12P19/20 ; A01P1/00 ; C12N9/16 ; C12N9/08 ; C12N9/20 ; C12N9/22 ; C11D3/386 ; D06M16/00 ; A23L1/0522 ; B08B3/04
摘要:
Variants of Bacillus sp. no. 707 alpha amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
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