Variants of an alpha-amylase with improved production levels in fermentation processes
    2.
    发明授权
    Variants of an alpha-amylase with improved production levels in fermentation processes 有权
    在发酵过程中提高生产水平的α-淀粉酶的变体

    公开(公告)号:US08546121B2

    公开(公告)日:2013-10-01

    申请号:US12601759

    申请日:2008-05-29

    IPC分类号: C12N9/26

    摘要: Variants of Bacillus sp. no. 707 alpha amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.

    摘要翻译: 芽孢杆菌的变种 没有。 提供707α淀粉酶,其更有效地并因此更经济地产生。 通过引入促进变体在发酵液中的溶解度的氨基酸变异来实现更高的发酵产率。 增加的溶解度允许在宿主细胞中表达后更多的酶保留在溶液中。 这又提高了从发酵液中回收表达的变体酶的效率。

    VARIANTS OF THE BACILLUS LICHENIFORMIS ALPHA-AMYLASE
    3.
    发明申请
    VARIANTS OF THE BACILLUS LICHENIFORMIS ALPHA-AMYLASE 审中-公开
    BACILLUS LICHENIFORMIS ALPHA-AMYLASE的变体

    公开(公告)号:US20110033882A1

    公开(公告)日:2011-02-10

    申请号:US12600818

    申请日:2008-05-28

    摘要: Variants of B. licheniformis alpha-amylase advantageously exhibit improved enzymatic performance. Suitable variants include those with an altered charge distribution on the surface of the enzyme or with altered active site residues. Structural modeling can inform the choice of amino acid modifications so that modified amino acids correspond to residues found in more active alpha amylases, for example. Compositions comprising the variants are useful in methods of cleaning surfaces, laundering textiles, desizing, treating starch, e.g., liquefaction and saccharification, and hydrolyzing biofilms off various substrates.

    摘要翻译: 地衣芽孢杆菌α-淀粉酶的变体有利地表现出改善的酶性能。 合适的变体包括在酶表面具有改变的电荷分布或具有改变的活性位点残基的变体。 结构建模可以指示氨基酸修饰的选择,使得修饰的氨基酸对应于例如在更有活性的α淀粉酶中发现的残基。 包含变体的组合物可用于清洁表面,洗涤纺织品,脱浆,处理淀粉,例如液化和糖化,以及从各种底物上水解生物膜的方法。

    Modified variant bowman birk protease inhibitors
    8.
    发明授权
    Modified variant bowman birk protease inhibitors 有权
    改良型bowman birk蛋白酶抑制剂

    公开(公告)号:US07803902B2

    公开(公告)日:2010-09-28

    申请号:US12252062

    申请日:2008-10-15

    IPC分类号: C07K14/00

    CPC分类号: C07K14/811 A61K8/64 A61Q7/02

    摘要: The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.

    摘要翻译: 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白(BBPI),并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体,表达和产生修饰变体BBPI的转化宿主细胞,修饰变体BBPI蛋白,包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。