Invention Application
- Patent Title: CRISP-SEQ, AN INTEGRATED METHOD FOR MASSIVELY PARALLEL SINGLE CELL RNA-SEQ AND CRISPR POOLED SCREENS
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Application No.: US16330774Application Date: 2017-09-14
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Publication No.: US20190194653A1Publication Date: 2019-06-27
- Inventor: Ido AMIT , Diego JAITIN , David LARA-ASTIASO , Assaf WEINER , Ido YOFE
- Applicant: Yeda Research and Development Co. Ltd.
- Applicant Address: IL Rehovot
- Assignee: Yeda Research and Development Co. Ltd.
- Current Assignee: Yeda Research and Development Co. Ltd.
- Current Assignee Address: IL Rehovot
- International Application: PCT/IL2017/051043 WO 20170914
- Main IPC: C12N15/11
- IPC: C12N15/11 ; C12N15/10 ; C12N9/22 ; C12Q1/6883
Abstract:
An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.
Public/Granted literature
- US11485971B2 CRISP-seq, an integrated method for massively parallel single cell RNA-seq and CRISPR pooled screens Public/Granted day:2022-11-01
Information query
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