Invention Application
- Patent Title: EMBEDDING MEDIUM OF BIOLOGICAL SAMPLE FOR ITS IMAGING BY LIGHT AND/OR ELECTRON MICROSCOPY
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Application No.: US16980560Application Date: 2019-03-15
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Publication No.: US20210003487A1Publication Date: 2021-01-07
- Inventor: Xavier HEILIGENSTEIN
- Applicant: CRYOCAPCELL
- Applicant Address: FR Paris
- Assignee: CRYOCAPCELL
- Current Assignee: CRYOCAPCELL
- Current Assignee Address: FR Paris
- Priority: EP18305293.5 20180316
- International Application: PCT/EP2019/056584 WO 20190315
- Main IPC: G01N1/36
- IPC: G01N1/36 ; C08F265/06 ; C08K5/098 ; H01J37/20 ; G02B21/33

Abstract:
The present invention relates to the field of microscopy, preferably electron microscopy. Especially, the present invention concerns an embedding medium for imaging a biological sample by microscopy comprising: from 60% to 99% wt. of a glycol dimethacrylate selected from alkylene glycol dimethacrylate and/or oligo(alkylene glycol) dimethacrylate; from 0% to 38% wt. of a polyalkylene glycol diacrylate or of a polyalkylene glycol methacrylate; said polyalkylene glycol diacrylate or polyalkylene glycol methacrylate being optionally substituted by at least one hydrophilic group such as hydroxyl, amino, or an oxo group; at least one additive, preferably comprising at least one heavy metal salt or lanthanide salt; and from 0.1% to 2% wt. of a radical polymerization initiator. The present invention also refers to the electro-conductive material resulting from the polymerization of the embedding medium of the invention, and the process and kits of preparation thereof; said material embedding at least one biological sample.
The present invention also relates to a method for imaging by microscopy, a biological sample comprising using the embedding medium and/or the electro-conductive material of the invention.
The present invention also relates to a method for imaging by microscopy, a biological sample comprising using the embedding medium and/or the electro-conductive material of the invention.
Public/Granted literature
- US11860074B2 Embedding medium of biological sample for its imaging by light and/or electron microscopy Public/Granted day:2024-01-02
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