- 专利标题: Method for Producing Dental Pulp-Derived Cells
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申请号: US17262302申请日: 2019-07-30
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公开(公告)号: US20210301258A1公开(公告)日: 2021-09-30
- 发明人: Kiwamu Imagawa , Kotaro Minami , Kenichi Maeda , Yuki Hosoda , Shunsuke Watanabe , Kazutoshi Sato , Yasuna Higashiguchi , Takashi Kushida , Ayumi Ishiwari
- 申请人: JCR Pharmaceuticals Co., Ltd. , Teijin Limited
- 申请人地址: JP Hyogo; JP Osaka-shi, Osaka
- 专利权人: JCR Pharmaceuticals Co., Ltd.,Teijin Limited
- 当前专利权人: JCR Pharmaceuticals Co., Ltd.,Teijin Limited
- 当前专利权人地址: JP Hyogo; JP Osaka-shi, Osaka
- 优先权: JP2018-143793 20180731
- 国际申请: PCT/JP2019/029913 WO 20190730
- 主分类号: C12N5/0775
- IPC分类号: C12N5/0775 ; C12N5/074 ; A01N1/02 ; A61K35/32 ; A61K35/545
摘要:
The present disclosure relates to a method for producing dental pulp-derived cells enriched with pluripotent stem cells including: (a) digesting dental pulp with a protease to prepare a dental pulp suspension; (b) culturing the suspension to proliferate pluripotent stem cells contained in the suspension; (c) freezing the proliferated pluripotent stem cells in a state in which the pluripotent stem cells are suspended in a first cryopreservation liquid; (d) thawing the frozen pluripotent stem cells; (e) culturing the thawed pluripotent stem cells in a state in which the pluripotent stem cells are adhered to surfaces of particles to proliferate the pluripotent stem cells on the surfaces of the particles; and (f) bringing the particles into contact with a protease to separate the pluripotent stem cells adhered to the surfaces of the particles from the particles.