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公开(公告)号:US20210301258A1
公开(公告)日:2021-09-30
申请号:US17262302
申请日:2019-07-30
发明人: Kiwamu Imagawa , Kotaro Minami , Kenichi Maeda , Yuki Hosoda , Shunsuke Watanabe , Kazutoshi Sato , Yasuna Higashiguchi , Takashi Kushida , Ayumi Ishiwari
IPC分类号: C12N5/0775 , C12N5/074 , A01N1/02 , A61K35/32 , A61K35/545
摘要: The present disclosure relates to a method for producing dental pulp-derived cells enriched with pluripotent stem cells including: (a) digesting dental pulp with a protease to prepare a dental pulp suspension; (b) culturing the suspension to proliferate pluripotent stem cells contained in the suspension; (c) freezing the proliferated pluripotent stem cells in a state in which the pluripotent stem cells are suspended in a first cryopreservation liquid; (d) thawing the frozen pluripotent stem cells; (e) culturing the thawed pluripotent stem cells in a state in which the pluripotent stem cells are adhered to surfaces of particles to proliferate the pluripotent stem cells on the surfaces of the particles; and (f) bringing the particles into contact with a protease to separate the pluripotent stem cells adhered to the surfaces of the particles from the particles.