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公开(公告)号:US20210301258A1
公开(公告)日:2021-09-30
申请号:US17262302
申请日:2019-07-30
发明人: Kiwamu Imagawa , Kotaro Minami , Kenichi Maeda , Yuki Hosoda , Shunsuke Watanabe , Kazutoshi Sato , Yasuna Higashiguchi , Takashi Kushida , Ayumi Ishiwari
IPC分类号: C12N5/0775 , C12N5/074 , A01N1/02 , A61K35/32 , A61K35/545
摘要: The present disclosure relates to a method for producing dental pulp-derived cells enriched with pluripotent stem cells including: (a) digesting dental pulp with a protease to prepare a dental pulp suspension; (b) culturing the suspension to proliferate pluripotent stem cells contained in the suspension; (c) freezing the proliferated pluripotent stem cells in a state in which the pluripotent stem cells are suspended in a first cryopreservation liquid; (d) thawing the frozen pluripotent stem cells; (e) culturing the thawed pluripotent stem cells in a state in which the pluripotent stem cells are adhered to surfaces of particles to proliferate the pluripotent stem cells on the surfaces of the particles; and (f) bringing the particles into contact with a protease to separate the pluripotent stem cells adhered to the surfaces of the particles from the particles.
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公开(公告)号:US10335439B2
公开(公告)日:2019-07-02
申请号:US14772854
申请日:2014-03-09
发明人: Kiwamu Imagawa , Kenichi Maeda , Yuki Hosoda , Shuichi Yokoyama , Naoki Okumura , Noriko Koizumi , Shigeru Kinoshita
摘要: [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate.[Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-β receptor inhibitor in various combinations.
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