Invention Application
- Patent Title: ENZYMATIC HEXOSAMINIDATION OF LACTOSE
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Application No.: US17416480Application Date: 2019-12-09
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Publication No.: US20220259580A1Publication Date: 2022-08-18
- Inventor: Kai-Uwe Baldenius , Michael Breuer , Corinna Ruffer , Melanie Weingarten , Reinhard Zschoche , Stefan Seemayer , Bernd Nidetzky , Katharina Schmoelzer , Michael Puhl
- Applicant: BASF SE
- Applicant Address: DE Ludwigshafen
- Assignee: BASF SE
- Current Assignee: BASF SE
- Current Assignee Address: DE Ludwigshafen
- Priority: EP18214455.0 20181220
- International Application: PCT/EP2019/084216 WO 20191209
- Main IPC: C12N9/24
- IPC: C12N9/24 ; C12P19/14 ; C12P19/18 ; C12N1/20

Abstract:
The present disclosure relates to methods for producing human milk oligosaccharide (HMO) core structures using glycosidases from family GH20 hexosaminidases. In particular, the present disclosure provides methods for producing lacto-N-triose II (LNT II) and/or lacto-N-tetraose (LNT) by reacting glucosamine-oxazoline and/or lacto-N-biose-oxazoline with lactose catalysed by an enzyme of the glycoside hydrolase family 20 (GH20) according to the classification of the Carbohydrate-Active-Enzymes (CAZy) database. Specific optimized enzymes are identified to catalyse the reactions.
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