公开(公告)号：US20220259580A1

公开(公告)日：2022-08-18

申请号：US17416480

申请日：2019-12-09

申请人： BASF SE

发明人： Kai-Uwe Baldenius ,  Michael Breuer ,  Corinna Ruffer ,  Melanie Weingarten ,  Reinhard Zschoche ,  Stefan Seemayer ,  Bernd Nidetzky ,  Katharina Schmoelzer ,  Michael Puhl

IPC分类号： C12N9/24 ,  C12P19/14 ,  C12P19/18 ,  C12N1/20

摘要： The present disclosure relates to methods for producing human milk oligosaccharide (HMO) core structures using glycosidases from family GH20 hexosaminidases. In particular, the present disclosure provides methods for producing lacto-N-triose II (LNT II) and/or lacto-N-tetraose (LNT) by reacting glucosamine-oxazoline and/or lacto-N-biose-oxazoline with lactose catalysed by an enzyme of the glycoside hydrolase family 20 (GH20) according to the classification of the Carbohydrate-Active-Enzymes (CAZy) database. Specific optimized enzymes are identified to catalyse the reactions.