Invention Publication
- Patent Title: PREPARATION AND USE OF PLANT EMBRYO EXPLANTS FOR TRANSFORMATION
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Application No.: US18071348Application Date: 2022-11-29
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Publication No.: US20230159942A1Publication Date: 2023-05-25
- Inventor: Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
- Applicant: Monsanto Technology LLC
- Applicant Address: US MO St. Louis
- Assignee: Monsanto Technology LLC
- Current Assignee: Monsanto Technology LLC
- Current Assignee Address: US MO St. Louis
- The original application number of the division: US17124115 2020.12.16
- Main IPC: C12N15/82
- IPC: C12N15/82 ; C12N5/00 ; A01H4/00 ; C12N5/04 ; G01N33/00 ; C12N9/10
Abstract:
The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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