公开(公告)号：US20240117366A1

公开(公告)日：2024-04-11

申请号：US18481464

申请日：2023-10-05

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  C12N9/10 ,  G01N33/00

CPC classification number: C12N15/8209 ,  A01H4/003 ,  A01H4/008 ,  C12N5/0025 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8201 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8221 ,  C12N15/8265 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  G01N33/0098 ,  A01H6/202 ,  C12N15/8261

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20220119826A1

公开(公告)日：2022-04-21

申请号：US17520352

申请日：2021-11-05

Applicant: Monsanto Technology LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Depperman ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

公开(公告)号：US20190382777A1

公开(公告)日：2019-12-19

申请号：US16393833

申请日：2019-04-24

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20180312854A1

公开(公告)日：2018-11-01

申请号：US15977229

申请日：2018-05-11

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Junjiang Shen ,  Edward Williams

IPC: C12N15/82 ,  G01N33/50 ,  C12R1/41

Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

公开(公告)号：US10006035B2

公开(公告)日：2018-06-26

申请号：US15151383

申请日：2016-05-10

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Junjiang Shen ,  Edward Williams

IPC: C12N15/84 ,  C12R1/41 ,  C12N15/82 ,  G01N33/50

CPC classification number: C12N15/8202 ,  C12N15/8205 ,  C12R1/41 ,  G01N33/5097

Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

公开(公告)号：US20250137002A1

公开(公告)日：2025-05-01

申请号：US18983993

申请日：2024-12-17

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  A01H6/20 ,  A01H6/46 ,  A01H6/54 ,  A01H6/60 ,  C12N1/20 ,  C12N5/00 ,  C12N5/04 ,  C12N9/10 ,  C12R1/41 ,  G01N33/00

Abstract: The present invention relates to methods and compositions for transforming soybean, corn. cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US11542514B2

公开(公告)日：2023-01-03

申请号：US17124115

申请日：2020-12-16

Applicant: Monsanto Technology LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Deppermann ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10 ,  A01H6/20 ,  A01H6/54 ,  A01H6/46 ,  A01H6/60 ,  C12N1/20 ,  C12R1/41

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

公开(公告)号：US09365859B2

公开(公告)日：2016-06-14

申请号：US14298739

申请日：2014-06-06

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Junjiang Shen ,  Edward Williams

IPC: C12N15/82 ,  C12R1/41

CPC classification number: C12N15/8202 ,  C12N15/8205 ,  C12R1/41 ,  G01N33/5097

Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

Abstract translation: 本发明涉及用于根瘤菌介导的植物细胞遗传转化的方法，包括大豆，卡诺拉油菜，玉米和棉花细胞。 这些包括依赖于VirD2和与VirD2无关的方法。 使用的细菌种类包括根瘤菌属，中华根瘤菌属（Sinorhizobium sp。）和中生根瘤菌属（Mesorhizobium sp。 还公开了用于这种转化的载体。

公开(公告)号：US20150184170A1

公开(公告)日：2015-07-02

申请号：US14642671

申请日：2015-03-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Deppermann ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/04

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Abstract translation: 本发明涉及从植物组织培养和遗传转化中切除包含来自种子的分生组织的外植体材料，以及这些材料的储存。 公开了组织制备，储存和转化的方法，以及通过这些方法生产的可转化的分生组织，以及组织制备。

公开(公告)号：US09006513B2

公开(公告)日：2015-04-14

申请号：US13750977

申请日：2013-01-25

Applicant: Monsanto Technology LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Deppermann ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: A01H1/00 ,  C12N15/82 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Abstract translation: 本发明涉及从植物组织培养和遗传转化中切除包含来自种子的分生组织的外植体材料，以及这些材料的储存。 公开了组织制备，储存和转化的方法，以及通过这些方法生产的可转化的分生组织，以及组织制备。