发明公开
- 专利标题: METHOD FOR INDUCING SALMONELLA ENTERITIDIS INTO VBNC STATE BY SODIUM HYPOCHLORITE
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申请号: US18352719申请日: 2023-07-14
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公开(公告)号: US20240018466A1公开(公告)日: 2024-01-18
- 发明人: Wen Wang , Yingping Xiao , Hua Yang , Xingning Xiao , Wentao LV , Lingyan Ma , Kun Pang
- 申请人: Zhejiang Academy of Agricultural Sciences , China Jiliang University
- 申请人地址: CN Hangzhou
- 专利权人: Zhejiang Academy of Agricultural Sciences,China Jiliang University
- 当前专利权人: Zhejiang Academy of Agricultural Sciences,China Jiliang University
- 当前专利权人地址: CN Hangzhou
- 优先权: CN 2210841210.8 2022.07.18
- 主分类号: C12N1/36
- IPC分类号: C12N1/36 ; C12N1/20 ; C12N1/02 ; C12Q1/04
摘要:
The disclosure discloses a method for inducing Salmonella enteritidis into VBNC state by sodium hypochlorite. The disclosure relates to the field of biotechnology, including preparing culture medium, which is BHI culture medium, and the culture medium is cooled to room temperature, and sterilized by ultraviolet for 15 min. The strain to be tested is incubated in BHI at 37° C. for 24 h in a sterile environment, and the bacterial cells are collected by centrifugation, washed with 0.85% (w/v) sterile physiological saline for 3 times, and then resuspended in 0.85% (w/v) sterile physiological saline to obtain 109 CFU/mL bacterial suspension. The bacteria cells are treated with sodium hypochlorite stress and the VBNC cells are counted.
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