公开(公告)号：US20240018466A1

公开(公告)日：2024-01-18

申请号：US18352719

申请日：2023-07-14

申请人： Zhejiang Academy of Agricultural Sciences ,  China Jiliang University

发明人： Wen Wang ,  Yingping Xiao ,  Hua Yang ,  Xingning Xiao ,  Wentao LV ,  Lingyan Ma ,  Kun Pang

IPC分类号： C12N1/36 ,  C12N1/20 ,  C12N1/02 ,  C12Q1/04

CPC分类号： C12N1/36 ,  C12N1/20 ,  C12N1/02 ,  C12Q1/04 ,  C12R2001/42

摘要： The disclosure discloses a method for inducing Salmonella enteritidis into VBNC state by sodium hypochlorite. The disclosure relates to the field of biotechnology, including preparing culture medium, which is BHI culture medium, and the culture medium is cooled to room temperature, and sterilized by ultraviolet for 15 min. The strain to be tested is incubated in BHI at 37° C. for 24 h in a sterile environment, and the bacterial cells are collected by centrifugation, washed with 0.85% (w/v) sterile physiological saline for 3 times, and then resuspended in 0.85% (w/v) sterile physiological saline to obtain 109 CFU/mL bacterial suspension. The bacteria cells are treated with sodium hypochlorite stress and the VBNC cells are counted.