A virucidal composition, a method of its manufacturing, related uses and end-user products are hereby provided. The composition includes a mixture of biologically active substances obtainable from selected fungal strains of Ganoderma lucidum (MUS12, MUS15, MUS18 and MUS23) by culturing the fungi in essentially liquid culture medium in a culture tank or a bioreactor in conditions essentially excluding agitation. The biologically active substances are obtained as a product excreted by mycelium of the above indicated G. lucidum strains and contain secondary metabolites, such as ganoderic acids and their derivatives. The composition has proved efficient in preventing the spread of viral infections. The composition can be used as a disinfecting agent for skin and nonliving surfaces and/or as an ingredient in preparation of personal care products.